大叶落地生根组氨醇脱氢酶基因克隆及表达分析
发布时间：2018-11-15 11:14
【摘要】：组氨醇脱氢酶将组氨醇氧化成组氨酸,是组氨酸合成途径中的最后一步,调控植物的生长和发育过程。为了更好地理解大叶落地生根中胎生苗调控的分子机制,利用RACE-PCR技术从大叶落地生根中克隆了1个新的组氨醇脱氢酶基因KdHDH。该基因编码的蛋白质具有483个氨基酸残基,分子量为52 130,等电点为5.78,其开放阅读框长度为1 452 bp。KdHDH蛋白与葡萄、拟南芥、蓖麻等植物的蛋白相似性都很高,聚类分析结果表明该蛋白与甜菜和菠菜的遗传距离最近。实时荧光定量PCR分析结果表明,该蛋白基因在大叶落地生根的根中表达量最高,且受渗透胁迫(甘露醇)的诱导下调表达。
[Abstract]:Histidine dehydrogenase oxidizes histidine to histidine which is the last step in histidine synthesis and regulates the growth and development of plants. In order to better understand the molecular mechanism of embryogenic seedling regulation in root formation, a new histamine dehydrogenase gene (KdHDH.) was cloned from the rooting of large leaf landroot by RACE-PCR technique. The protein encoded by this gene has a molecular weight of 52,130 and a isoelectric point of 5.78. The open reading frame length of 1 452 bp.KdHDH protein is similar to that of grape, Arabidopsis thaliana, castor and other plants. The results of cluster analysis showed that the genetic distance between the protein and beet and spinach was the closest. The results of real-time fluorescence quantitative PCR analysis showed that the expression of the protein gene was the highest in the rooting roots, and the expression was down-regulated by osmotic stress (mannitol).
【作者单位】： 深圳市日f园林绿化有限公司;暨南大学深圳旅游学院;北京林业大学林学院草坪研究所;华南农业大学林学与风景园林学院草业科学系/广东省草业工程技术研究中心;
【基金】：深圳市知识创新项目(基20160093) 北京林业大学与厦门日懋城建园林建设股份有限公司产学研合作项目
【分类号】：Q943.2
[Abstract]:Histidine dehydrogenase oxidizes histidine to histidine which is the last step in histidine synthesis and regulates the growth and development of plants. In order to better understand the molecular mechanism of embryogenic seedling regulation in root formation, a new histamine dehydrogenase gene (KdHDH.) was cloned from the rooting of large leaf landroot by RACE-PCR technique. The protein encoded by this gene has a molecular weight of 52,130 and a isoelectric point of 5.78. The open reading frame length of 1 452 bp.KdHDH protein is similar to that of grape, Arabidopsis thaliana, castor and other plants. The results of cluster analysis showed that the genetic distance between the protein and beet and spinach was the closest. The results of real-time fluorescence quantitative PCR analysis showed that the expression of the protein gene was the highest in the rooting roots, and the expression was down-regulated by osmotic stress (mannitol).
【作者单位】： 深圳市日f园林绿化有限公司;暨南大学深圳旅游学院;北京林业大学林学院草坪研究所;华南农业大学林学与风景园林学院草业科学系/广东省草业工程技术研究中心;
【基金】：深圳市知识创新项目(基20160093) 北京林业大学与厦门日懋城建园林建设股份有限公司产学研合作项目
【分类号】：Q943.2
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