基于线粒体COⅡ基因的中国大豆食心虫不同地理种群遗传分化分析
发布时间:2018-11-15 15:36
【摘要】:【目的】大豆食心虫Leguminivora glycinivorella(Matsumura)是我国大豆Glycina max最重要的蛀荚害虫。本研究旨在探讨中国大豆食心虫不同地理种群的遗传分化情况。【方法】通过测定大豆食心虫19个地理种群208头老熟幼虫的线粒体COⅡ基因序列(723 bp),利用MEGA6.0和Dna SP 5.0等软件分析大豆食心虫不同地理种群的基因序列变异和遗传分化情况。【结果】在19个大豆食心虫地理种群中共获得208条COⅡ基因序列,发现61个变异位点,包含57个单倍型。总群体单倍型多样度Hd=0.8522,各地理种群单倍型多样度范围在0.1818~0.9546间,总群体的固定系数Fst=0.4619,总遗传分化系数Gst=0.1186,总基因流Nm=0.29。总群体Tajima’s D检验结果不显著,表明大豆食心虫在较近的历史时期未出现群体扩张现象,群体大小稳定。单倍型网络图、ML单倍型系统树及采用不同地理种群间遗传距离构建的UPGMA系统发育树均显示,贵阳(GY)和都安(DA)种群与其余地理种群分化明显。AMOVA分子变异分析结果显示,东北组、西北组、黄淮组、华南组及西南组种群组间所占总变异的比例大于组内。【结论】大豆食心虫不同地理种群间基因交流较少,整体的遗传多样性和遗传分化程度较高,遗传分化主要来自地理种群组间,且地理距离是影响不同地理种群间遗传距离的重要因素。
[Abstract]:Objective: soybean borer Leguminivora glycinivorella (Matsumura) is the most important pod borer of soybean Glycina max in China. The purpose of this study was to investigate the genetic differentiation of different populations of soybean heart-eating insects in China. [methods] Mitochondrial CO 鈪,
本文编号:2333702
[Abstract]:Objective: soybean borer Leguminivora glycinivorella (Matsumura) is the most important pod borer of soybean Glycina max in China. The purpose of this study was to investigate the genetic differentiation of different populations of soybean heart-eating insects in China. [methods] Mitochondrial CO 鈪,
本文编号:2333702
本文链接:https://www.wllwen.com/kejilunwen/jiyingongcheng/2333702.html
最近更新
教材专著