不同致病力棉花黄萎病菌乙烯含量及相关基因表达量的测定

发布时间：2018-11-16 15:36

【摘要】：为了分析大丽轮枝菌(Verticillium dahliae Kleb.)乙烯合成与致病力的关系,本研究以棉花黄萎病菌落叶型强致病力菌株V592和非落叶型弱致病力菌株V774为研究对象,分别将2个菌株于非诱导查氏培养液中和添加棉花根提取物的诱导查氏培养液中进行培养,对其乙烯含量进行测定,并通过qRT-PCR分析大丽轮枝菌中6个与乙烯合成相关基因的表达量。结果表明:强、弱致病力菌株均能产生乙烯,强致病力菌株产生的乙烯含量明显高于弱致病力菌株,且强致病力菌株合成乙烯的能力受寄主根诱导后明显加强。非诱导条件下4个基因在强、弱致病力菌株中的表达量存在差异;在诱导条件下6个基因在强、弱致病力菌株中的表达量存在明显差异(P0.05)。这些基因的差异表达可能是造成强、弱致病力菌株乙烯含量不同的主要因素。
[Abstract]:To analyze (Verticillium dahliae Kleb.) of Verticillium dahliensis The relationship between ethylene synthesis and pathogenicity was studied by using virulent virulence strain V592 and non-deciduous virulence strain V774 of Verticillium wilt. The two strains were cultured in uninduced Charing's culture medium and in the medium supplemented with cotton root extract. The ethylene content of the two strains was determined. The expression of 6 genes related to ethylene synthesis was analyzed by qRT-PCR. The results showed that both strong and weak pathogenicity strains could produce ethylene, and the ethylene content of strong pathogenic strains was significantly higher than that of weak pathogenicity strains, and the ability of strong pathogenicity strains to synthesize ethylene was obviously enhanced after induced by host roots. There were significant differences in the expression of four genes in strong and weak virulence strains under non-induced conditions, and in strong and weak virulence strains in induced condition (P0.05). The differential expression of these genes may be the main factor of the different ethylene content of strong and weak virulence strains.
【作者单位】：石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室;
【基金】：国家自然科学基金项目(31160351、31560494、31460451)
【分类号】：S435.621.2

【相似文献】