葡萄果实成熟期花色苷与白藜芦醇合成相关基因的表达

发布时间：2018-11-16 16:12

【摘要】：以酿酒葡萄‘赤霞珠’为试材,采用pH示差法和高效液相色谱(HPLC)法分别测定葡萄成熟期果皮花色苷和白藜芦醇含量,用实时荧光定量PCR检测两者合成途径中相关基因的表达量,分析花色苷含量和白藜芦醇含量与其相关基因表达的关系,以揭示结构基因与调控基因的调控机制,为筛选富含花色苷和白藜芦醇的酿酒葡萄提供理论依据。结果显示:(1)葡萄果皮花色苷含量在花后112d达到最高值(0.77mg/g),反式白藜芦醇含量在花后126d达到最高值(30.87μg/g)。(2)花色苷和白藜芦醇合成途径中,CHSs、CHI、STS、UFGT、MybA1、MybA2基因的表达量除花后98d下调外,其余时间均呈上调表达,而Myb5a则始终呈上调表达。(3)相关分析表明,STS基因表达量与CHS1、CHS2基因表达量呈极显著和显著正相关关系,MybA1、MybA2基因表达量与CHSs、CHI、STS、UFGT基因的表达量呈极显著正相关关系;Myb5a基因表达量与CHS3基因表达量呈极显著正相关关系。研究表明,部分结构基因的表达与花色苷和白藜芦醇的变化不同步,MybA1和MybA2可能调控花色苷合成途径中多个结构基因的表达,花色苷与白藜芦醇的关系并不固定,而是处在动态变化中。
[Abstract]:The content of anthocyanin and resveratrol in grape peel of wine grape 'Cabernet Sauvignon' was determined by pH differential method and high performance liquid chromatography (HPLC). The expression of genes related to anthocyanin and resveratrol was detected by real-time fluorescence quantitative PCR, and the relationship between anthocyanin content and resveratrol content was analyzed to reveal the regulatory mechanism of structural gene and regulatory gene. To provide theoretical basis for screening wine grape rich in anthocyanin and resveratrol. The results showed that: (1) the anthocyanin content of grape peel reached the highest value (0.77mg/g) at 112d after anthesis, and the content of trans-resveratrol reached the highest value (30.87 渭 g / g). (_ 2) after anthesis (30.87 渭 g / g). (_ 2), and the content of trans-resveratrol reached the highest value (30.87 渭 g / g). (_ 2) in the synthesis pathway of resveratrol. The expression of CHSs,CHI,STS,UFGT,MybA1,MybA2 gene was up-regulated at 98 days after anthesis, while that of Myb5a was up-regulated. (3) correlation analysis showed that the expression of STS gene was correlated with that of CHS1,. The expression of CHS2 gene was significantly and positively correlated with the expression of CHSs,CHI,STS,UFGT gene, and the expression of MybA1,MybA2 gene was positively correlated with the expression of CHSs,CHI,STS,UFGT gene. There was a significant positive correlation between Myb5a gene expression and CHS3 gene expression. The results showed that the expression of some structural genes was not synchronized with the changes of anthocyanin and resveratrol. MybA1 and MybA2 might regulate the expression of multiple structural genes in anthocyanin synthesis pathway, and the relationship between anthocyanin and resveratrol was not fixed. But in dynamic change.
【作者单位】：宁夏大学农学院;宁夏大学葡萄与葡萄酒教育部工程中心;
【基金】：国家自然科学基金地区科学基金(31260456)
【分类号】：S663.1

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