广西巴马小型猪PPARγ基因克隆及组织表达差异分析

发布时间：2018-11-18 12:05

【摘要】：【目的】克隆广西巴马小型猪过氧化物酶增殖物激活受体γ基因(PPARγ),并确定其在不同组织中的表达情况,为后续研究该基因在猪支原体肺炎(MPS)中的作用机制打下基础。【方法】利用RT-PCR克隆广西巴马小型猪PPARγ基因,并进行BLAST比对分析及其推导蛋白的生物信息学分析;以实时荧光定量PCR检测PPARγ基因在18月龄广西巴马小型猪心脏、脾脏、肺脏、肾脏、大肠、小肠、肌肉和脂肪中的表达情况。【结果】广西巴马小型猪PPARγ基因开放阅读框序列1515 bp,编码504个氨基酸。广西巴马小型猪PPARγ基因推导氨基酸序列与Gen Bank已发表的猪PPARγ基因(FJ436399.1)推导氨基酸序列同源性最高,达100.0%;而与牛(NM_181024.2)、人类(NM_005037.5)、猕猴(NM_001032860.1)、鼠(NM_001308354.1)和鸿雁(KJ019822.1)的同源性分别为92.9%、91.8%、91.5%、90.5%和83.5%,表明PPARγ基因高度保守。PPARγ蛋白分子量为57380.91 Da,理论等电点(p I)为6.88,不稳定系数49.26,脂肪指数为88.21,亲水性平均水平为-0.293,为亲水性蛋白,其二级结构主要是α-螺旋,占总数的39.09%。PPARγ基因在广西巴马小型猪大肠组织中的表达量最高,极显著高于其他组织(P0.01),而在肾脏、心脏和肌肉组织中的表达量极低。【结论】PPARγ基因在18月龄广西巴马小型猪的各组织中均有表达,但不同组织中的表达水平存在明显差异,可能与其在不同组织中的功能差异有关。
[Abstract]:[objective] to clone the peroxidase proliferator activated receptor 纬 gene (PPAR 纬) from Guangxi Bama miniature pig and to determine its expression in different tissues. [methods] RT-PCR was used to clone the PPAR 纬 gene of Guangxi Bama miniature pig, and the BLAST comparison analysis and the bioinformatics analysis of the deduced protein were carried out. Real-time fluorescence quantitative PCR was used to detect PPAR 纬 gene in the heart, spleen, lung, kidney, large intestine and small intestine of 18-month-old Guangxi Bama miniature pig. Expression of PPAR 纬 gene in muscle and fat. [results] 1515 bp, encoding 504 amino acids in PPAR 纬 gene of Guangxi Bama miniature pig. The deduced amino acid sequence of PPAR 纬 gene of Guangxi Bama miniature pig was the highest homology with the deduced amino acid sequence of PPAR 纬 gene (FJ436399.1) published by Gen Bank. The homology with NM_181024.2, NM_005037.5, NM_001032860.1, NM_001308354.1 and KJ019822.1 was 92.91.8and 91.5, respectively. 90.5% and 83.5%, indicating that the PPAR 纬 gene is highly conserved, the molecular weight of PPAR 纬 protein is 57380.91 Da, the theoretical isoelectric point (p I) is 6.88, the coefficient of instability is 49.26, the fat index is 88.21, and the average hydrophilic level is -0.293. It is a hydrophilic protein, and its secondary structure is mainly 伪 -helix. The expression of 39.09%.PPAR 纬 gene in the large intestine of Guangxi Bama miniature pig is the highest, which is significantly higher than that in other tissues (P0.01), but in the kidney. The expression of PPAR 纬 gene was very low in heart and muscle tissue. [conclusion] PPAR 纬 gene was expressed in all tissues of 18-month-old Guangxi Bama miniature pig, but there were significant differences in expression level between different tissues. It may be related to the functional differences in different tissues.
【作者单位】：广西大学动物科学技术学院;
【基金】：国家现代农业产业技术体系广西生猪创新团队建设项目(nycytxgxcxtd-03-15) 广西家畜遗传育种改良重点实验室开放项目(2015GXKLLGI-09)
【分类号】：Q78;S858.28

【相似文献】