MiRNA-520e对HeLa细胞FOXL2基因的靶向调控作用及细胞迁移增殖的影响

发布时间：2018-11-19 10:01

【摘要】：目的探讨miRNA-520e对人宫颈癌HeLa细胞FOXL2基因的调控作用及对细胞迁移、增殖的影响。方法应用生物信息学分析软件(Target Scan Human 7.1)预测miRNA-520e可能结合于FOXL2 mRNA 3'UTR端,以HeLa细胞作为试验研究对象,将细胞随机分为阴性对照组(加入无关序列NC Fam mimics)、正常对照组(不加任何干预)、miRNA-520e组(加入miRNA-520e mimics)。运用脂质体法通过Lipofectamine 3000 Reagent将各miRNA mimics转染各组HeLa细胞,48 h后通过实时荧光定量PCR及Western blotting法检测FOXL2 mRNA及蛋白表达;转染24 h后采用细胞划痕试验检测细胞迁移能力,使用ACEA xCELLigence RTCA DP细胞分析仪检测细胞增殖能力。结果 miRNA-520e组HeLa细胞FOXL2 mRNA和蛋白的表达量较正常对照组及阴性对照组降低,且miRNA-520e组细胞的迁移和增殖能力较正常对照组及阴性对照组增强(P均0.05)。结论 miRNA-520e可抑制HeLa细胞FOXL2 mRNA和蛋白的表达,促进细胞迁移和增殖。
[Abstract]:Objective to investigate the effect of miRNA-520e on the regulation of FOXL2 gene and cell migration and proliferation in human cervical cancer HeLa cells. Methods the bioinformatics software (Target Scan Human 7.1 was used to predict the possible binding of miRNA-520e to the FOXL2 mRNA 3'UTR terminal. HeLa cells were randomly divided into negative control group (adding the unrelated sequence NC Fam mimics),). Normal control group (without any intervention), miRNA-520e group (add miRNA-520e mimics).) The miRNA mimics was transfected into HeLa cells by Lipofectamine 3000 Reagent with liposome method. After 48 h, the expression of FOXL2 mRNA and protein was detected by real-time fluorescence quantitative PCR and Western blotting. After 24 hours of transfection, the cell migration ability was detected by cell scratch test, and the cell proliferation ability was detected by ACEA xCELLigence RTCA DP cell analyzer. Results the expression of FOXL2 mRNA and protein in HeLa cells in miRNA-520e group was lower than that in normal control group and negative control group, and the cell migration and proliferation ability in miRNA-520e group was higher than that in normal control group and negative control group (P 0.05). Conclusion miRNA-520e can inhibit the expression of FOXL2 mRNA and protein in HeLa cells and promote cell migration and proliferation.
【作者单位】：潍坊医学院;
【基金】：国家自然科学基金资助项目(81272122)
【分类号】：R737.33

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