刺五加FPS基因DNA序列和启动子的克隆与分析

发布时间：2018-11-21 17:30

【摘要】：为获得刺五加FPS的DNA和启动子序列,以及其功能元件信息和基因结构,根据已克隆的FPS cDNA序列设计引物,使用TAIL-PCR技术克隆FPS的基因组DNA及启动子序列,再通过各生物软件分析基因结构与启动子功能元件。得到刺五加FPS基因全长为7 952 bp,含有12个外显子和11个内含子,启动子序列含有25个类型功能元件,包括:62个TATA-box、24个CAAT-box,以及其他类型的重要功能元件34个。本研究首次克隆得到刺五加FPS的DNA序列和启动子全长,获取了内外显子的分布情况以及启动子功能元件,为后续进一步研究FPS在刺五加中的表达调控奠定了基础。
[Abstract]:In order to obtain the DNA and promoter sequence of Acanthopanax senticosus FPS and its functional element information and gene structure, primers were designed according to the cloned FPS cDNA sequence and the genomic DNA and promoter sequences of FPS were cloned by TAIL-PCR technique. Then the gene structure and promoter functional elements were analyzed by various biological software. The total length of Acanthopanax senticosus FPS gene was 7 952 bp, with 12 exons and 11 introns. The promoter sequence contained 25 functional elements, including 62 TATA-box, 24 CAAT-box, and 34 other important functional elements. In this study, the DNA sequence and promoter length of Acanthopanax senticosus FPS were cloned for the first time, and the distribution of exon and promoter functional elements were obtained, which laid a foundation for further study on the expression and regulation of FPS in Acanthopanax senticosus (Acanthopanax senticosus).
【作者单位】：华北理工大学生命科学学院;
【基金】：国家自然科学基金项目(31570683) 河北省教育厅资助科研项目(QN2014102) 华北理工大学研究生创新项目(2017S12);华北理工大学培育基金项目(SP201508)共同资助
【分类号】：Q943.2;S567.19

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