黄鳝ghrelin基因的克隆及分子特征分析

发布时间：2018-11-22 20:30

【摘要】：Ghrelin是联系生殖和能量代谢的重要桥梁信号分子,通过克隆黄鳝(Monopterus albus)的ghrelin基因并对其基因结构和功能进行了初步分析;运用c DNA末端快速扩增技术获得了黄鳝ghrelin基因的c DNA序列和DNA序列全长。结果表明,黄鳝ghrelin基因c DNA全长552 bp(Gen Bank accession no.JX122807),包括115 bp的5'端非编码区、324 bp的完整开放阅读框以及113 bp的3'端非编码区;DNA序列全长1323 bp,由3个内含子和4个外显子构成,内含子剪切位点具有典型识别核苷酸GT/AG,3个内含子分别为594 bp、84 bp和93 bp,4个外显子长度分别为229 bp、78 bp、112 bp和133 bp。氨基酸序列分析显示,ghrelin基因推导的Ghrelin蛋白前体原(propreghrelin)序列由26 aa的信号肽、19 aa的成熟肽以及C端氨基酸残基等构成;其中,成熟肽第3位为丝氨酸(Ser3),是Ghrelin的酰基化位点;C端氨基酸残基序列极可能包括与Ghrelin成熟肽功能相互拮抗的肥胖抑制素(Obestatin)。氨基酸的同源性及进化关系分析表明,黄鳝与某些鲈形目鱼类的蛋白前体原存在高度相似性,且在进化上黄鳝与较高级的鲈形目、鲽形目鱼类聚为一支。ghrelin基因结构及其蛋白质某些氨基酸残基序列的高度保守,预示着Ghrelin在脊椎动物中有着重要的生理功能与类似的作用机制。
[Abstract]:Ghrelin is an important bridge signal molecule related to reproduction and energy metabolism. The ghrelin gene of Monopterus Albus (Monopterus albus) was cloned and its gene structure and function were preliminarily analyzed. The full length of c DNA and DNA sequences of ghrelin gene of Monopterus Albus were obtained by rapid amplification of c DNA terminal. The results showed that the c DNA of Monopterus Albus was 552 bp (Gen Bank accession no.JX122807 in length, including the 5 '-terminal noncoding region of 115 bp, the complete open reading frame of 324 bp and the 3' -terminal non-coding region of 113 bp. The total length of DNA 1323 bp, consists of 3 introns and 4 exons. The intron splicing site has typical recognition nucleotide, GT/AG, 3 introns are 594 bp,84 bp and 93 bp, exons are 229bp,78 bp, respectively. 112 bp and 133 bp. Amino acid sequence analysis showed that the precursor (propreghrelin) sequence of Ghrelin protein derived from ghrelin gene was composed of signal peptide of 26 aa, mature peptide of 19 aa and C-terminal amino acid residues. The third position of mature peptide is serine (Ser3), which is the acylation site of Ghrelin, and the C-terminal amino acid residues probably include (Obestatin)., which antagonizes the function of Ghrelin mature peptide. The analysis of amino acid homology and evolution showed that the protein precursor of Monopterus Albus was highly similar to that of some Perciformes, and it was evolutionarily similar to the higher order Perciformes. The structure of ghrelin gene and some amino acid residues of its protein are highly conserved, indicating that Ghrelin has important physiological functions and similar mechanisms in vertebrates.
【作者单位】：长江大学动物科学学院湿地生态与农业利用教育部工程研究中心;农业部淡水渔业和种质资源利用重点实验室中国水产科学研究院淡水渔业研究中心;淡水水产健康养殖湖北省协同创新中心;
【基金】：湖北省自然科学基金(2013CFB393) 农业部淡水渔业与种质资源利用重点实验室开放基金课题(KF201307) 国家支撑计划课题(2013BAD20B06) 湖北省支撑计划项目(2015BBA235) 湖北省高等学校优秀中青年创新团队项目(T201503)
【分类号】：S917.4

【相似文献】