水稻G蛋白γ亚基基因RGG2调控株型和抗旱性的研究
发布时间：2018-11-23 19:18
【摘要】：异三聚体G蛋白(Heterotrimeric G protein),是由a,p和γ三个亚单位构成的蛋白质复合物,它是生物体内一类重要的信号蛋白,参与多个信号响应过程。水稻中共有4个G蛋白γ亚基,分别为RGG1、RGG2、GS3和qPE9-1/DEP1,其中GS3是一个控制籽粒大小的主效QTL,它在调节籽粒和器官大小中发挥负调节子的功能；而qPE9-1是水稻直立穗形成的关键基因,同时参与水稻氮素和ABA的响应。而另外两个G蛋白γ亚基RGGI和RGG2尚未进行详细的功能分析。本研究以RGG2为研究对象,对该基因进行了表达分析、亚细胞定位和组织形态学等分析,初步揭示了RGG2的生物学功能。主要结果如下：1、RGG2属于B型G蛋白γ亚基,与RGB1之间存在明显互作；2、RGG2基因为组成型表达,且其表达水平比水稻其它3个G蛋白γ亚基基因RGG1、GS3和qPE9-1高出10倍以上。亚细胞定位结果显示,RGG2蛋白位于细胞核、细胞质和细胞膜上；3、过量表达RGG2基因导致植株矮化,穗和叶片变短、直立,籽粒短圆,粒重减少,单株产量下降。说明RGG2是这些性状的负调控因子;4、扫描电镜和组织解剖分析表明,RGG2过量表达通过抑制细胞纵向伸长,而使得籽粒变小、节间变短,但不影响细胞数目。细胞分裂相关基因的表达水平在野生型和转基因株系中差异很小,也从侧面验证了这一结论；5、观察了日本晴和RGG2过量表达株系在ABA胁迫下的种子萌发与幼苗生长情况,发现RGG2过量表达系对ABA的响应显著增强：6.干旱处理表明,RGG2过量表达株系叶片离体失水率和干旱胁迫下的植株死亡率均显著低于对照。以上结果说明,RGG2作为G蛋白γ亚基,不仅调控水稻株型、粒型,还参与水稻对ABA及抗旱胁迫响应。
[Abstract]:Heterotrimer G protein (Heterotrimeric G protein),) is a protein complex composed of three subunits of ACP and 纬. It is a kind of important signal protein in organism and participates in many signal response processes. There are four G protein 纬 subunits in rice, RGG1,RGG2,GS3 and qPE9-1/DEP1, respectively. GS3 is a major QTL, that controls grain size. It plays a negative role in regulating grain and organ size. QPE9-1 is the key gene of rice panicle formation, and it is involved in the response of rice nitrogen and ABA. The other two G protein 纬 subunits, RGGI and RGG2, have not been analyzed in detail. In this study, the expression, subcellular localization and histomorphology of RGG2 gene were analyzed, and the biological function of RGG2 was preliminarily revealed. The main results are as follows: 1RGG2 belongs to type B G protein 纬 subunit, and there is obvious interaction between RGG2 and RGB1; (2) the expression level of RGG2 gene was 10 times higher than that of other three G protein 纬 subunit genes, RGG1,GS3 and qPE9-1. The results of subcellular localization showed that RGG2 protein was located in nucleus, cytoplasm and cell membrane, and 3, overexpression of RGG2 gene resulted in plant dwarfing, short panicle and leaf, erect, short round grain, reduced grain weight and decreased yield per plant. The results showed that RGG2 was a negative regulatory factor for these traits, and (4) scanning electron microscopy and histological analysis showed that RGG2 overexpression decreased the grain and shortened the internodes, but did not affect the number of cells by inhibiting the lengthwise elongation of the cells. The expression level of cell division related genes in wild type and transgenic lines was very small, and this conclusion was verified from the side. 5. Seed germination and seedling growth of Nippon and RGG2 overexpression lines under ABA stress were observed. It was found that the response of RGG2 overexpression lines to ABA was significantly enhanced: 6. Drought treatment showed that the rate of water loss in leaves of RGG2 overexpression lines and the plant mortality under drought stress were significantly lower than those of the control. These results suggest that RGG2, as a G protein 纬 subunit, not only regulates the plant type and grain type of rice, but also participates in the response of rice to ABA and drought stress.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S511
本文编号：2352465
[Abstract]:Heterotrimer G protein (Heterotrimeric G protein),) is a protein complex composed of three subunits of ACP and 纬. It is a kind of important signal protein in organism and participates in many signal response processes. There are four G protein 纬 subunits in rice, RGG1,RGG2,GS3 and qPE9-1/DEP1, respectively. GS3 is a major QTL, that controls grain size. It plays a negative role in regulating grain and organ size. QPE9-1 is the key gene of rice panicle formation, and it is involved in the response of rice nitrogen and ABA. The other two G protein 纬 subunits, RGGI and RGG2, have not been analyzed in detail. In this study, the expression, subcellular localization and histomorphology of RGG2 gene were analyzed, and the biological function of RGG2 was preliminarily revealed. The main results are as follows: 1RGG2 belongs to type B G protein 纬 subunit, and there is obvious interaction between RGG2 and RGB1; (2) the expression level of RGG2 gene was 10 times higher than that of other three G protein 纬 subunit genes, RGG1,GS3 and qPE9-1. The results of subcellular localization showed that RGG2 protein was located in nucleus, cytoplasm and cell membrane, and 3, overexpression of RGG2 gene resulted in plant dwarfing, short panicle and leaf, erect, short round grain, reduced grain weight and decreased yield per plant. The results showed that RGG2 was a negative regulatory factor for these traits, and (4) scanning electron microscopy and histological analysis showed that RGG2 overexpression decreased the grain and shortened the internodes, but did not affect the number of cells by inhibiting the lengthwise elongation of the cells. The expression level of cell division related genes in wild type and transgenic lines was very small, and this conclusion was verified from the side. 5. Seed germination and seedling growth of Nippon and RGG2 overexpression lines under ABA stress were observed. It was found that the response of RGG2 overexpression lines to ABA was significantly enhanced: 6. Drought treatment showed that the rate of water loss in leaves of RGG2 overexpression lines and the plant mortality under drought stress were significantly lower than those of the control. These results suggest that RGG2, as a G protein 纬 subunit, not only regulates the plant type and grain type of rice, but also participates in the response of rice to ABA and drought stress.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S511
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