HSP22过表达介导线粒体未折蛋白反应对PD转基因果蝇的影响
发布时间：2018-11-24 19:36
【摘要】：目的:探讨HSP22过表达介导线粒体未折叠蛋白反应对PD转基因果蝇的影响,为进一步研究PD的致病机制提供依据。方法:采用HSP22 OE、GCN2RNAi和平衡系HSP22 OE;GCN2 RNAi基因干预PD转基因果蝇,通过飞行试验观察对果蝇表型的影响,蛋白免疫印迹检测P-AMPK、P-e IF2α表达水平。结果:GCN2 RNAi能明显降低PD转基因果蝇异翅率(P0.005),轻微提高果蝇的跳跃或者飞行能力(P0.05),使PD转基因果蝇的P-AMPK和P-e IF2α表达水平均下调;HSP22 OE使PD转基因果蝇的异翅率显著降低(P0.001),伴随着跳跃或者飞行能力显著提高(P0.001),P-AMPK和P-e IF2α表达水平均显著下调;平衡系HSP22OE;GCN2 RNAi明显降低PD转基因果蝇异翅率(P0.005),轻微提高跳跃或者飞行能力(P0.05),反而使PD转基因果蝇的P-AMPK和P-e IF2α表达水平上调。结论:(1)UPRmt过度应激水平是PD转基因果蝇的致病机制之一。(2)HSP22对PD转基因果蝇具有保护作用,其保护机制可能与UPRmt应激水平存在负相关性。
[Abstract]:Aim: to investigate the effect of HSP22 overexpression mediated mitochondrial unfolded protein reaction on PD transgenic Drosophila melanogaster, and to provide evidence for further study on the pathogenesis of PD. Methods: HSP22 OE,GCN2RNAi and balance line HSP22 OE;GCN2 RNAi gene were used to interfere with PD transgenic Drosophila. The phenotype of Drosophila melanogaster was observed by flight test. The expression of P-AMPKG P-e IF2 伪 was detected by Western blot. Results: GCN2 RNAi could significantly decrease the abnormal wing rate of PD transgenic Drosophila (P0.005), slightly improve the jumping or flying ability of Drosophila melanogaster (P0.05), and down-regulate the expression of P-AMPK and P-e IF2 伪 in PD transgenic Drosophila (P0.05). HSP22 OE significantly decreased the heteropterygoid rate of PD transgenic Drosophila (P0. 001), accompanied by jumping or flight ability (P0. 001), and decreased the expression of P-AMPK and P-e IF2 伪. GCN2 RNAi significantly decreased the abnormal wing rate of PD transgenic Drosophila (P0.005), slightly improved the jumping or flying ability (P0.05), but increased the expression of P-AMPK and P-e IF2 伪 in PD transgenic Drosophila (P0.05). Conclusion: (1) the level of UPRmt overstress is one of the pathogenetic mechanisms of PD transgenic Drosophila. (2) HSP22 has protective effect on PD transgenic Drosophila, and its protective mechanism may have a negative correlation with UPRmt stress level.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R741
本文编号：2354788
[Abstract]:Aim: to investigate the effect of HSP22 overexpression mediated mitochondrial unfolded protein reaction on PD transgenic Drosophila melanogaster, and to provide evidence for further study on the pathogenesis of PD. Methods: HSP22 OE,GCN2RNAi and balance line HSP22 OE;GCN2 RNAi gene were used to interfere with PD transgenic Drosophila. The phenotype of Drosophila melanogaster was observed by flight test. The expression of P-AMPKG P-e IF2 伪 was detected by Western blot. Results: GCN2 RNAi could significantly decrease the abnormal wing rate of PD transgenic Drosophila (P0.005), slightly improve the jumping or flying ability of Drosophila melanogaster (P0.05), and down-regulate the expression of P-AMPK and P-e IF2 伪 in PD transgenic Drosophila (P0.05). HSP22 OE significantly decreased the heteropterygoid rate of PD transgenic Drosophila (P0. 001), accompanied by jumping or flight ability (P0. 001), and decreased the expression of P-AMPK and P-e IF2 伪. GCN2 RNAi significantly decreased the abnormal wing rate of PD transgenic Drosophila (P0.005), slightly improved the jumping or flying ability (P0.05), but increased the expression of P-AMPK and P-e IF2 伪 in PD transgenic Drosophila (P0.05). Conclusion: (1) the level of UPRmt overstress is one of the pathogenetic mechanisms of PD transgenic Drosophila. (2) HSP22 has protective effect on PD transgenic Drosophila, and its protective mechanism may have a negative correlation with UPRmt stress level.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R741
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