2012~2015年H9N2亚型AIV分离株HA基因的克隆及序列分析

发布时间：2018-11-28 12:55

【摘要】：为了解近年来中国部分地区H9N2亚型禽流感病毒流行特点及遗传进化情况,利用RT-PCR方法扩增2012~2015年分离的17株H9N2亚型禽流感病毒的HA基因片段,并进行序列测定和遗传进化分析,同时对HA蛋白的裂解位点、受体结合位点和潜在的糖基化位点进行分析。结果显示,17株H9N2亚型禽流感病毒HA基因核苷酸和推导的氨基酸同源性分别为87%~100%和75%~100%,均属于Y280-like亚系毒株。HA基因裂解位点均为非连续碱性氨基酸,属于低致病力毒株。HA基因受体结合位点149、198、234和235位氨基酸存在变异,其中,16株分离毒株的234位氨基酸由Q突变为L,表现出人流感病毒受体结合特征。潜在糖基化位点分析结果显示,11株病毒在218位氨基酸处缺失1个糖基化位点,4株病毒在492位氨基酸处缺失1个糖基化位点,17株病毒在313位氨基酸处增加1个糖基化位点。研究结果表明,应加强对H9N2亚型AIV的流行病学监测,关注疫苗毒株与流行毒株的差异。
[Abstract]:In order to understand the epidemic characteristics and genetic evolution of H9N2 subtype avian influenza virus in some parts of China in recent years, the HA gene fragments of 17 strains of H9N2 subtype avian influenza virus isolated from 2012 to 2015 were amplified by RT-PCR method. At the same time, the cleavage sites, receptor binding sites and potential glycosylation sites of HA protein were analyzed. The results showed that the nucleotide sequence and deduced amino acid homology of HA gene of 17 strains of H9N2 subtype avian influenza virus were 87% and 75%, respectively, which belonged to Y280-like substrain. The HA gene cleavage sites were all discontinuous basic amino acids. The HA receptor binding sites of 149198234 and 235 amino acids were mutated, and the amino acids of 16 isolated strains were mutated from Q to L, showing the receptor binding characteristics of human influenza virus. The results of potential glycosylation site analysis showed that 11 strains lacked one glycosylation site at 218 amino acids, 4 lacked one glycosylation site at 492 amino acids, and 17 increased one glycosylation site at 313 amino acids. The results showed that the epidemiological monitoring of AIV subtype of H9N2 should be strengthened and the differences between vaccine strains and epidemic strains should be paid attention to.
【作者单位】：广州市华南农大生物药品有限公司;
【基金】：广州市产学研协同创新重大专项(201508020088)
【分类号】：S852.65

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