胸腺瘤中E-cadherin、β-catenin蛋白和基因的表达

发布时间：2018-11-28 18:53

【摘要】：目的探讨E-cadherin、β-catenin蛋白和基因的表达与胸腺瘤侵袭性的相关性。方法收集2012年~2016年东莞市人民医院存档的胸腺瘤手术标本120例,参照胸腺瘤镜下表现、Masaoka临床分期标准、临床表现及影像资料将其分为无侵袭性和有侵袭性两组,并对两组分别行E-cadherin和β-catenin抗体免疫组化染色及E-cadherin和β-catenin基因荧光原位杂交实验。结果有侵袭性胸腺瘤55例,无侵袭性胸腺瘤65例,E-cadherin和β-catenin蛋白在无侵袭性和有侵袭性胸腺瘤中的表达差异有统计学意义(P0.05)。在无侵袭性和有侵袭性胸腺瘤中,E-cadherin基因表达缺失和β-catenin基因表达扩增组间差异均有统计学意义(P0.05)。结论应用免疫组化检测E-cadherin和β-catenin蛋白及荧光原位杂交技术检测E-cadherin和β-catenin基因,有助于无侵袭性和有侵袭性胸腺瘤的鉴别。
[Abstract]:Objective to investigate the relationship between the expression of E-cadherin, 尾-catenin protein and gene and the invasiveness of thymoma. Methods 120 cases of thymoma were collected from 2012 to 2016 in Dongguan people's Hospital. According to the findings of thymoma, Masaoka clinical staging standard, clinical manifestation and imaging data, they were divided into two groups: noninvasive group and invasive group. Immunohistochemical staining of E-cadherin and 尾-catenin antibodies and fluorescence in situ hybridization of E-cadherin and 尾-catenin genes were performed in the two groups. Results there were 55 cases of invasive thymoma and 65 cases of non-invasive thymoma. The expression of E-cadherin and 尾-catenin protein in noninvasive thymoma and invasive thymoma were significantly different (P0.05). In noninvasive thymoma and invasive thymoma, there were significant differences in E-cadherin gene expression deletion and 尾-catenin gene expression amplification (P0.05). Conclusion Immunohistochemical detection of E-cadherin and 尾-catenin protein and fluorescence in situ hybridization to detect E-cadherin and 尾-catenin genes may be helpful in differentiating noninvasive thymoma from invasive thymoma.
【作者单位】：广东省东莞市人民医院病理科;
【分类号】：R736.3

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