水稻条纹病毒对介体灰飞虱生长发育相关基因转录水平的影响

发布时间：2018-12-06 07:47

【摘要】：由水稻条纹病毒(Rice stripe virus,RSV)引起的水稻条纹叶枯病是我国粳稻产区的一种毁灭性病毒病害。RSV主要由半翅目昆虫灰飞虱(Laodelphax striatellus)以持久增殖型方式传播,与无毒灰飞虱相比,带毒灰飞虱五龄和整个若虫阶段的发育历期缩短,最终羽化的成虫数减少。为了从分子上揭示RSV对灰飞虱生长发育的影响,本文研究了RSV对灰飞虱生长发育相关基因转录水平的影响。将灰飞虱转录组拼接后建立本地数据库,根据黑腹果蝇生长发育基因序列比对出10种灰飞虱同源基因序列,设计特异引物,通过RT-q PCR方法分别对带毒和无毒灰飞虱体内这10种基因的转录水平进行检测。结果显示,在RSV的影响下,8种基因的转录发生显著变化,其中2种蛋白质转录被激活,6种蛋白质受到抑制。动力蛋白和钠钾转移ATP酶转录水平的变化可能影响灰飞虱神经系统发育,造成昆虫行为异常;桩蛋白转录下降会影响翅的发育;与细胞骨架相关的5种蛋白质转录也受到了RSV抑制,可能影响灰飞虱羽化过程。对生长发育基因转录水平的初步探究为更好地理解RSV与灰飞虱的互作奠定了基础。
[Abstract]:Rice stripe leaf blight caused by rice stripe virus (Rice stripe virus,RSV) is a devastating virus disease in japonica rice growing areas in China. RSV is mainly transmitted by (Laodelphax striatellus), a hemiptera insect, in a long-lasting proliferative manner. Compared with non-toxic ash planthopper, the development period of the fifth instar and the whole nymph stage of the virulent fly planthopper was shortened and the number of adult emergence was decreased. In order to reveal the molecular effect of RSV on the growth and development of planthopper, the effect of RSV on the transcription level of genes related to the growth and development of planthopper was studied in this paper. The local database was established by splicing the transcriptome of fly, and 10 homologous gene sequences of ash planthopper were compared according to the growth and development gene sequence of Drosophila melanogaster, and specific primers were designed. The transcriptional levels of these 10 genes in virulent and nontoxic fly planthopper were detected by RT-q PCR. The results showed that under the influence of RSV, the transcription of 8 genes changed significantly, of which 2 proteins were activated and 6 proteins were inhibited. The changes of ATP transcriptional level may affect the development of nervous system of planthopper, cause insect behavior abnormality, decrease of pile protein transcription will affect the development of wings. The transcription of five proteins associated with cytoskeleton was also inhibited by RSV, which may affect the emergence process of planthopper. The preliminary study on the transcriptional level of growth and development genes laid a foundation for a better understanding of the interaction between RSV and ash planthopper.
【作者单位】：中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室;
【基金】：国家自然科学基金资助项目(31401713)
【分类号】：S435.11

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