陆地棉微管结合蛋白CLASP家族基因的鉴定及表达分析

发布时间：2018-12-06 17:23

【摘要】：CLASP蛋白(CLIP-associated protein,CLASP)是一种调节微管结构与功能的微管结合蛋白,在植物生长发育及形态建成过程中起着至关重要的作用。本研究利用生物信息学的方法,在陆地棉基因组数据库中鉴定出6个编码CLASP蛋白的基因。多重序列比对及系统进化树分析表明,陆地棉CLASPs可分为2个亚家族(I和II),亚家族I含有HEAT重复结构域和典型的CLASP-N端结构域,亚家族II只含有CLASP-N端结构域。实时荧光定量PCR结果表明,6个CLASPs基因在棉花各组织中均有表达,但表达模式各不相同,其中CotAD_63740(Gen Bank登录号为KX881965)和CotAD_04861(Gen Bank登录号为KX881961)在纤维中优势表达,CotAD_48232(Gen Bank登录号为KX881962)、CotAD_48665(Gen Bank登录号为KX881963)、CotAD_55570(Gen Bank登录号为KX881964)和CotAD_68468(Gen Bank登录号为KX881966)在茎中优势表达,表达模式的不同表明CLASPs在棉花不同组织中功能不同。本研究为深入研究棉花CLASPs的功能奠定了基础。
[Abstract]:CLASP protein (CLIP-associated protein,CLASP) is a microtubule-binding protein that regulates the structure and function of microtubules and plays an important role in plant growth and morphogenesis. In this study, six genes encoding CLASP protein were identified in the genome database of upland cotton by bioinformatics. Multiple sequence alignment and phylogenetic tree analysis showed that CLASPs of upland cotton could be divided into two subfamilies (I and II), subfamily I) containing HEAT repeat domain and typical CLASP-N terminal domain, and II only contained CLASP-N terminal domain. The results of real-time fluorescence quantitative PCR showed that all of the six CLASPs genes were expressed in different tissues of cotton, but the expression patterns were different. Among them, CotAD_63740 (Gen Bank accession number was KX881965) and CotAD_04861 (Gen Bank accession number was KX881961). CotAD_48232 (Gen Bank login number is KX881962), CotAD_48665 (Gen Bank login number is KX881963), CotAD_55570 (Gen Bank login number is KX881964) and CotAD_68468 (Gen Bank login number is KX881966). Different expression patterns indicated that CLASPs had different functions in different tissues of cotton. This study laid a foundation for further study on the function of cotton CLASPs.
【作者单位】：石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室;
【基金】：国家自然科学基金项目(U1128301) 新疆生产建设兵团博士资金专项(2013BB003) 石河子大学高层次人才启动项目(RCZX201218)资助~~
【分类号】：S562

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