胸腺移植合并CD4~--DLI对异基因造血干细胞移植后T细胞重建的影响

发布时间：2018-12-11 13:17

【摘要】：目的研究胸腺移植(TT)合并CD4~--DLI对异基因造血干细胞移植(allo-HSCT)后T细胞重建的影响。方法将BALB/c小鼠随机分成3组,单纯造血干细胞移植(HSCT组),造血干细胞移植+胸腺移植(TT组),造血干细胞移植~+胸腺移植+去CD4~+T细胞的供体淋巴细胞输注(CD4~--DLI组)。移植前1 d,对小鼠进行致死剂量的放疗处理。移植当天,进行造血干细胞移植,供体为C57BL/6小鼠。移植处理后5 d、2周、4周、3个月分别处死小鼠,取小鼠外周血和脾脏细胞进行流式细胞术检测T细胞表面抗原,T细胞受体,初始T细胞,细胞内因子;HE染色观察供体胸腺的发育情况。结果 TT和CD4~--DLI不会影响彼此对T细胞重建的作用;TT合并CD4~--DLI能够通过胸腺依赖和胸腺非依赖两种途径加快CD4~+和CD8~+T细胞数目的重建、扩大初始CD4~+和CD8~+T细胞库、加快T细胞功能的重建,并且对TCR库和胸腺没有副作用。结论:TT合并CD4~--DLI可以加快allo-HSCT后T细胞重建。
[Abstract]:Objective to study the effect of thymus transplantation (TT) combined with CD4~--DLI on T cell reconstruction after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods BALB/c mice were randomly divided into three groups: simple hematopoietic stem cell transplantation (HSCT group), hematopoietic stem cell transplantation thymus transplantation (TT group). Hematopoietic stem cell transplantation ~ thymus transplantation donor lymphocyte infusion of CD4~ T cells (CD4~--DLI group). The mice were treated with lethal dose of radiotherapy one day before transplantation. On the day of transplantation, hematopoietic stem cells were transplanted to C57BL/6 mice. Mice were killed 5 days after transplantation for 2 weeks, 4 weeks and 3 months respectively. Peripheral blood and spleen cells were collected for flow cytometry to detect T cell surface antigen, T cell receptor, initial T cell and intracellular factor. The development of donor thymus was observed by HE staining. Results TT and CD4~--DLI had no effect on T cell reconstruction. TT combined with CD4~--DLI can accelerate the reconstruction of CD4~ and CD8~ T cells by thymus dependence and thymus independence, expand the initial CD4~ and CD8~ T cell banks, and accelerate T cell function reconstruction, and has no side effects on TCR library and thymus. Conclusion: TT combined with CD4~--DLI can accelerate T cell reconstruction after allo-HSCT.
【作者单位】：广东医科大学东莞市环境医学重点实验室;南方医科大学南方医院小儿科;
【基金】：国家自然科学基金资助项目(编号:81270632) 广东省“扬帆计划”紧缺拔尖人才项目(编号:201433004) 留学人员科技活动项目择优资助项目(编号:2QT14002) 广东省普通高校特色创新项目(编号:2015KTSCX051)
【分类号】：R457.7

【相似文献】