拟南芥At5g66070基因在ABA处理下的初步研究
发布时间：2018-12-12 18:09
【摘要】：本研究以拟南芥为实验材料,探索基因At5g66070在植物激素ABA处理条件下的相关生理功能.结果表明,在10μMABA处理下,该基因的表达量明显升高,表明该基因受到ABA的诱导.亚细胞定位发现AT5G66070定位在细胞核.通过DNA及RNA水平筛选鉴定At5g66070基因T-DNA插入纯合突变体,然后经根瘤农杆菌介导法进行遗传转化,筛选获得过表达转基因株系.表型分析发现经ABA处理后,突变体相对于野生型而言根长较短,表明突变体对ABA更为敏感.气孔关闭实验发现10μM ABA能诱导突变体气孔关闭,而野生型和过表达无显著影响.以上结果表明At5g66070在拟南芥的ABA胁迫响应中起负调控作用.
[Abstract]:In this study, Arabidopsis thaliana was used as experimental material to explore the physiological function of gene At5g66070 under the condition of plant hormone ABA treatment. The results showed that the expression of the gene was significantly increased under the treatment of 10 渭 MABA, indicating that the gene was induced by ABA. Subcellular localization revealed that AT5G66070 was located in the nucleus. The At5g66070 gene T-DNA was inserted into homozygous mutants by DNA and RNA screening, and then transformed by Agrobacterium tumefaciens. Phenotypic analysis showed that the root length of the mutant was shorter than that of the wild type after ABA treatment, indicating that the mutant was more sensitive to ABA. Stomatal closure test showed that 10 渭 M ABA could induce stomatal closure of mutant, but wild type and overexpression had no significant effect. These results suggest that At5g66070 plays a negative role in the response to ABA stress in Arabidopsis thaliana.
【作者单位】： 四川大学生命科学学院生物资源与生态环境教育部重点实验室;
【基金】：转基因专项(2016ZX08009003-002-00)
【分类号】：Q943.2
，
本文编号：2375057
[Abstract]:In this study, Arabidopsis thaliana was used as experimental material to explore the physiological function of gene At5g66070 under the condition of plant hormone ABA treatment. The results showed that the expression of the gene was significantly increased under the treatment of 10 渭 MABA, indicating that the gene was induced by ABA. Subcellular localization revealed that AT5G66070 was located in the nucleus. The At5g66070 gene T-DNA was inserted into homozygous mutants by DNA and RNA screening, and then transformed by Agrobacterium tumefaciens. Phenotypic analysis showed that the root length of the mutant was shorter than that of the wild type after ABA treatment, indicating that the mutant was more sensitive to ABA. Stomatal closure test showed that 10 渭 M ABA could induce stomatal closure of mutant, but wild type and overexpression had no significant effect. These results suggest that At5g66070 plays a negative role in the response to ABA stress in Arabidopsis thaliana.
【作者单位】： 四川大学生命科学学院生物资源与生态环境教育部重点实验室;
【基金】：转基因专项(2016ZX08009003-002-00)
【分类号】：Q943.2
，
本文编号：2375057
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