巴西橡胶树SnRK1家族基因的克隆与表达分析

发布时间：2018-12-13 05:52

【摘要】：蔗糖非酵解型蛋白激酶I(Sn RK1)是植物糖信号途径的关键因子,广泛参与植物生长发育。以蓖麻Sn RK1基因序列为探针,采用同源克隆的方法获得了橡胶树Sn RK1家族2个基因的全长c DNA,分别命名为Hb Sn RK1-1(KX237690.1)和Hb Sn RK1-2(KX237691.1)。2个Hb Sn RK1基因均编码含515个氨基酸且序列高度一致(96.5%)的蛋白质;2个基因均含10个外显子、9个内含子,且外显子大小相近,但基因长度差异明显。在6种橡胶树组织中,2个Hb Sn RK1基因均有表达,但Hb Sn RK1-1在胶乳(产胶细胞乳管的细胞质)中的表达量明显高于Hb Sn RK1-2;在叶片发育不同时期,Hb Sn RK1-1的表达变化不显著,而Hb Sn RK1-2的表达随叶片发育明显下降;在胶乳中,Hb Sn RK1-1的表达显著受乙烯和茉莉酸诱导、受2,4-D抑制,但Hb Sn RK1-2表达受激素处理的影响较小,推测Hb Sn RK1可能参与胶乳再生的激素调控。
[Abstract]:Sucrose non-fermentative protein kinase (I (Sn RK1) is a key factor of sugar signaling pathway in plants and is widely involved in plant growth and development. The full-length c DNA, of two genes of Sn RK1 family of rubber tree was obtained by homologous cloning using Sn RK1 gene sequence of castor as probe. They are named Hb Sn RK1-1 (KX237690.1) and Hb Sn RK1-2 (KX237691.1) respectively. The two Hb Sn RK1 genes encode 515 amino acids and the sequence is highly consistent (96.5%). The two genes contained 10 exons and 9 introns, and the size of exons was similar, but the gene length was significantly different. Two Hb Sn RK1 genes were expressed in 6 kinds of Hevea, but the expression of Hb Sn RK1-1 in latex (cytoplasm of latex cells) was significantly higher than that in Hb Sn RK1-2;. The expression of, Hb Sn RK1-1 did not change significantly at different stages of leaf development, but the expression of Hb Sn RK1-2 decreased significantly with leaf development. The expression of, Hb Sn RK1-1 in latex was significantly induced by ethylene and jasmonic acid and inhibited by 24D, but the expression of Hb Sn RK1-2 was less affected by hormone treatment. It was suggested that Hb Sn RK1 might be involved in hormone regulation of latex regeneration.
【作者单位】：海南大学热带农林学院;中国热带农业科学院橡胶研究所;
【基金】：国家863项目(No.2013AA102605)
【分类号】：S794.1

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