2种非生物胁迫和7种激素对水稻OsAQP基因表达的影响
发布时间：2018-12-15 07:09
【摘要】：OsAQP是从cDNA文库中分离的一种新的水稻水通道蛋白基因,本实验室前期利用半定量RT-PCR技术发现该基因的表达与非生物胁迫、植物激素有关.本文采用qRT-PCR方法,检测了干旱、高盐胁迫以及ABA等7种植物激素对OsAQP在幼苗期水稻根和叶中表达的影响,结果显示干旱、高盐以及7种植物激素MeJA,SA,6-BA,GA,IAA,ABA,BR均能不同程度诱导OsAQP基因上调表达,在根中的诱导上调水平显著高于叶,提示该基因功能涉及生长发育、抗逆应答等多种生物学过程,且与水稻的根的关系更为密切.
[Abstract]:OsAQP is a new rice aquaporin gene isolated from cDNA library. The expression of this gene was found to be related to abiotic stress and plant hormones by semi-quantitative RT-PCR technique. The effects of seven plant hormones, such as drought, high salt stress and ABA, on the expression of OsAQP in roots and leaves of rice at seedling stage were detected by qRT-PCR method. The results showed that drought, high salt and 7 plant hormones MeJA,SA,6-BA,GA,IAA, ABA,BR could induce up-regulated expression of OsAQP gene in different degree, and the up-regulation level in root was significantly higher than that in leaves, suggesting that the function of OsAQP gene was involved in many biological processes, such as growth and development, stress resistance response and so on, and was more closely related to the root of rice.
【作者单位】： 河南师范大学生命科学学院;
【基金】：国家自然科学基金项目(31171182) 河南省高校科技创新团队(15IRTSTHN020)
【分类号】：Q943.2
本文编号：2380208
[Abstract]:OsAQP is a new rice aquaporin gene isolated from cDNA library. The expression of this gene was found to be related to abiotic stress and plant hormones by semi-quantitative RT-PCR technique. The effects of seven plant hormones, such as drought, high salt stress and ABA, on the expression of OsAQP in roots and leaves of rice at seedling stage were detected by qRT-PCR method. The results showed that drought, high salt and 7 plant hormones MeJA,SA,6-BA,GA,IAA, ABA,BR could induce up-regulated expression of OsAQP gene in different degree, and the up-regulation level in root was significantly higher than that in leaves, suggesting that the function of OsAQP gene was involved in many biological processes, such as growth and development, stress resistance response and so on, and was more closely related to the root of rice.
【作者单位】： 河南师范大学生命科学学院;
【基金】：国家自然科学基金项目(31171182) 河南省高校科技创新团队(15IRTSTHN020)
【分类号】：Q943.2
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