检测血浆中p16及CDO1基因甲基化在胶质瘤诊断中的价值

发布时间：2018-12-15 16:02

【摘要】：采用甲基化特异PCR(MSP)法分别检测49例胶质瘤患者组织及其对应的术前血浆,10例颅脑外伤患者挫伤的脑组织及其对应的术前血浆和16例健康体检者血浆中p16及半胱氨酸双加氧酶1(CDO1)基因的甲基化状态,以探讨血浆中检测p16及CDO1基因甲基化在胶质瘤临床诊断中的价值。胶质瘤组织中p16及CDO1基因甲基化率分别为53.1%(26/49)、42.9%(21/49),对应的血浆甲基化率为39.0%(19/49)、34.7%(17/49)。在肿瘤组织和血浆中p16及CDO1至少一个[p16和(或)CDO1]基因甲基化的频率为75.5%(37/49)和67.3%(33/49)。血浆中p16及CDO1基因(甲基化)联合检测胶质瘤的灵敏度为67.3%,较单基因检测的灵敏度明显提高。单因素分析显示术前患者血浆中p16和(或)CDO1基因的甲基化与年龄、性别、肿瘤分级无关。对一些肿瘤特异基因的甲基化状态进行联合检测可提高胶质瘤在血清学诊断水平上的灵敏度,为胶质瘤的筛查及早期诊断提供新的临床诊断思路。
[Abstract]:The tissues of 49 patients with glioma and their corresponding preoperative plasma were detected by methylation specific PCR (MSP). The methylation status of p16 and cysteine dioxygenase-1 (CDO1) gene in the brain tissue of 10 patients with craniocerebral trauma and their corresponding preoperative plasma and 16 healthy controls. To evaluate the clinical value of detection of p16 and CDO1 gene methylation in plasma for glioma. The methylation rates of p16 and CDO1 genes in glioma tissues were 53.1% (26 / 49) and 42.9% (21 / 49), respectively. The corresponding plasma methylation rates were 39.0% (19 / 49) and 34.7% (17 / 49), respectively. At least one [p16 and / or CDO1] gene methylation frequency of p16 and CDO1 in tumor tissue and plasma was 75.5% (37 / 49) and 67.3% (33 / 49). The sensitivity of combined detection of p16 and CDO1 gene (methylation) in plasma was 67.3, which was significantly higher than that of single gene. Univariate analysis showed that the methylation of p16 and / or CDO1 gene was not related to age, sex and tumor grade. The combined detection of methylation status of some tumor-specific genes can improve the sensitivity of the serological diagnosis of gliomas and provide a new clinical diagnosis method for the screening and early diagnosis of gliomas.
【作者单位】：安徽医科大学第一附属医院神经外科;安徽医科大学第四附属医院神经外科;
【基金】：安徽省科技攻关计划项目(编号:1301042208)
【分类号】：R739.41

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