绝经前后不同骨密度女性外周血单核细胞基因表达谱分析

发布时间：2018-12-16 17:49

【摘要】：目的分析绝经前后低骨密度、高骨密度女性外周血单核细胞基因表达谱,探讨外周血单核细胞差异表达基因对低骨密度、高骨密度人群的鉴别诊断价值。方法下载公众数据平台美国国家生物技术信息中心(National Center of Biotechnology Information,NCBI)下的基因芯片原始数据集NCBI-GEO Data Sets-GSE56815,运用R/Bionconductor分析原始数据,选取差异倍数1.5,P0.05的基因为差异表达基因;运用Medcalc及SPSS统计软件对差异表达基因绘制受试者工作特征曲线,分析单一基因检测及联合基因检测对低骨密度、高骨密度人群的鉴别诊断效果。结果在绝经前女性中,低骨密度、高骨密度组外周血单核细胞存在着58个差异基因(18基因上调、40基因下调),且FOLR3ITGB3ABOIGHM联合检测鉴别诊断不同骨密度女性有较好的特异性、敏感性;在绝经后女性中,低骨密度、高骨密度组外周血单核细胞存在着75个差异基因(21基因上调,54基因下调),且TACSTD2MMP8LILRA5联合检测鉴别诊断不同骨密度女性有较好的特异性、敏感性。结论绝经前后女性外周血单核细胞基因表达谱不同,低骨密度组与高骨密度组外周血单核细胞基因表达谱在绝经后女性中差异变化更为显著,外周血单核细胞差异基因联合检测能够提高对不同骨密度女性的鉴别诊断能力。
[Abstract]:Objective to analyze the gene expression profiles of peripheral blood monocytes in premenopausal and premenopausal women with low BMD and high BMD, and to explore the differential diagnosis of PBMC differentially expressed genes in patients with low BMD and high BMD. Methods the original data set of gene chip was downloaded from (National Center of Biotechnology Information,NCBI (National Biotechnology Information Center), a public data platform. R/Bionconductor was used to analyze the original data, and the multiple of difference was 1.5. P05 genes were differentially expressed genes. Medcalc and SPSS software were used to draw the operating characteristic curves of the differentially expressed genes, and to analyze the effect of single gene detection and combined gene detection on the differential diagnosis of low BMD and high BMD population. Results among premenopausal women, 58 differentially expressed genes (18 genes up-regulated, 40 genes down-regulated) were found in peripheral blood mononuclear cells with low BMD and high BMD, and the differential diagnosis of BMD in women with different BMD by FOLR3ITGB3ABOIGHM combined with BMD was more specific. Sensitivity; In postmenopausal women, 75 differentially expressed genes were found in peripheral blood monocytes of low BMD and high BMD groups (21 gene up-regulated, 54 down-regulated), and TACSTD2MMP8LILRA5 combined detection for differential diagnosis of different BMD women had better specificity and sensitivity. Conclusion the gene expression profiles of peripheral blood monocytes in premenopausal and postmenopausal women are different, and the gene expression profiles of peripheral blood monocytes in low BMD group and high BMD group are more significant in postmenopausal women. The differential gene detection of peripheral blood monocytes can improve the differential diagnosis ability of women with different bone mineral density.
【作者单位】：西安交通大学医学院附属红会医院检验科;电信科学技术第十研究所;
【基金】：西安市红会医院科研基金资助课题(YJ2016013)
【分类号】：R580;R440

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