温度和盐度变化对两株米氏凯伦藻生长及MAPK基因表达量影响的初步研究
发布时间：2018-12-18 03:25
【摘要】：温度和盐度分别作为海洋环境中基础又重要的环境因素之一,对海洋浮游微藻的生长有重要的影响。促有丝分裂活化蛋白激酶(mitogen-activated protein kinase,MAPK)是信号传导通路的关键分子,存在于真核生物中,它对于植物的生长发育调节及抗逆应答等生理过程起到了重要的作用。为了深入探讨有害藻华生物对温度和盐度变化的分子响应,本文以MAPK基因作为分析对象,以我国近海常见的有害藻华原因种米氏凯伦藻(Karenia mikimotoi)为研究目标。选择不同来源的两株米氏凯伦藻藻株:惠州株和福建株,分别培养在温度和盐度逐渐变化的环境中(温度的变化范围为15-33℃,盐度变化范围为15-38),从藻细胞的比生长速率、叶绿素a浓度、最大潜在光合能力及分子生物学等方面,探究两株米氏凯伦藻对于温度和盐度变化的适应性,以及温度和盐度变化对MAPK基因表达的影响。本研究取得的主要研究结果如下:1.米氏凯伦藻福建株的温度适应范围是15-30℃,盐度适应范围是20-37,最适温度范围是24-28℃,最适盐度范围是23-37。米氏凯伦藻惠州株的温度适应范围是15-33℃,盐度适应范围是20-37,最适温度范围是24-30℃,最适盐度范围是26-32。2.在温度变化实验中,米氏凯伦藻福建株在28℃时达到最大比生长速率,为0.37d~(-1);而惠州株在26℃时达到最大比生长速率,为0.31d~(-1)。在盐度变化实验中,米氏凯伦藻福建株在23时达到最大比生长速率,为0.34d~(-1),而米氏凯伦藻惠州株在26时达到最大比生长速率,为0.20d~(-1)。3.叶绿素a浓度和最大潜在光合能力Fv/Fm值均在一定程度上反映了两株米氏凯伦藻的生长状态。其中叶绿素a浓度与藻细胞密度成正相关关系。米氏凯伦藻福建株的Fv/Fm值在温度≥30℃时受到抑制,在盐度≥37和≤20时受到抑制。米氏凯伦藻惠州株的Fv/Fm值在温度≥28℃和≤20℃时受到抑制,在盐度在≥38和≤17时受到抑制。4.米氏凯伦藻惠州株在22和26℃时MAPK基因显著上调,在20和28℃时MAPK基因显著下调。福建株在15和33℃时MAPK基因显著下调,在20、26、28和30℃时MAPK基因显著上调。米氏凯伦藻惠州株在盐度30~37时MAPK基因显著下调,在盐度17和20时MAPK基因相对表达量显著上调。福建株在盐度23、26、30和32时MAPK基因显著上调,在34和37时MAPK基因显著下调。结果表明MAPK基因与米氏凯伦藻适应温度和盐度变化的调控相关,说明MAPK基因参与了对外界温度和盐度变化信号的转导过程。
[Abstract]:As one of the basic and important environmental factors in marine environment, temperature and salinity play an important role in the growth of marine planktonic microalgae. Mitogenic activated protein kinase (mitogen-activated protein kinase,MAPK) is a key signal transduction pathway in eukaryotes and plays an important role in plant growth and development regulation and stress resistance response. In order to study the molecular response of harmful algal blooms to temperature and salinity changes, MAPK gene was used as an analysis object, and (Karenia mikimotoi), a common species of harmful algal blooms, was used as the research object in this paper. Two strains of Karen Michaelis from different sources, Huizhou strain and Fujian strain, were cultured in a gradually changing environment of temperature and salinity (temperature range 15-33 鈩，
本文编号：2385252
[Abstract]:As one of the basic and important environmental factors in marine environment, temperature and salinity play an important role in the growth of marine planktonic microalgae. Mitogenic activated protein kinase (mitogen-activated protein kinase,MAPK) is a key signal transduction pathway in eukaryotes and plays an important role in plant growth and development regulation and stress resistance response. In order to study the molecular response of harmful algal blooms to temperature and salinity changes, MAPK gene was used as an analysis object, and (Karenia mikimotoi), a common species of harmful algal blooms, was used as the research object in this paper. Two strains of Karen Michaelis from different sources, Huizhou strain and Fujian strain, were cultured in a gradually changing environment of temperature and salinity (temperature range 15-33 鈩，
本文编号：2385252
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