脂肪酸对离体培养中华绒螯蟹肝胰腺PUFAs合成相关基因表达的影响

发布时间：2018-12-21 15:51

【摘要】：长期以来海水水生动物和淡水水生动物多不饱和脂肪酸合成能力一直是众多研究的重点。相比于淡水水生动物,海水水生动物更容易从食物中获得多不饱和脂肪酸,因此其合成多不饱和脂肪酸的能力低于淡水水生动物。中华绒螯蟹作为一种洄游型甲壳动物,其脂肪酸合成能力的研究显得更加重要。本研究通过中华绒螯蟹肝胰腺离体培养技术,利用不同的脂肪酸培养中华绒螯蟹肝胰腺组织,旨在探讨不同脂肪酸对中华绒螯蟹肝胰腺多不饱和脂肪酸合成相关基因表达的影响,为研究饲料中添加不同脂肪酸对中华绒螯蟹多不饱和脂肪酸合成的影响提供了一定的理论参考依据。本研究表明:油酸会显著增加中华绒螯蟹肝胰腺ELOVL6、fad6、和fad9基因的表达量;不同脂肪酸对fad6和fad9基因表达量的影响相似,且相比于EPA和DHA,亚麻酸和油酸会显著增加fad6和fad9基因的表达。通过本研究,我们得出EPA和DHA等多不饱和脂肪酸会抑制多不饱和脂肪酸合成的过程。进一步证实了海水水生动物多不饱和脂肪酸合成能力和海水中高含量的多不饱和脂肪酸之间的关系。
[Abstract]:For a long time, the synthesis ability of polyunsaturated fatty acids in seawater and freshwater aquatic animals has been the focus of many studies. Compared with freshwater aquatic animals, seawater aquatic animals are easier to obtain polyunsaturated fatty acids from food, so their ability to synthesize polyunsaturated fatty acids is lower than that of fresh water aquatic animals. As a migratory crustacean, the fatty acid synthesis ability of Eriocheir sinensis (Eriocheir sinensis) is more important. In this study, the hepatopancreas of Eriocheir sinensis were cultured in vitro with different fatty acids. To investigate the effects of different fatty acids on the expression of polyunsaturated fatty acid syntheses in the hepatopancreas of Eriocheir sinensis. It provides a theoretical basis for the study of the effects of different fatty acids on the synthesis of polyunsaturated fatty acids in Eriocheir sinensis (Eriocheir sinensis). The results showed that oleic acid could significantly increase the expression of ELOVL6,fad6, and fad9 genes in the hepatopancreas of Eriocheir sinensis. The effects of different fatty acids on the expression of fad6 and fad9 genes were similar, and the expression of fad6 and fad9 genes was significantly increased compared with EPA and DHA, linolenic acid and oleic acid. In this study, we concluded that polyunsaturated fatty acids, such as EPA and DHA, inhibit the synthesis of polyunsaturated fatty acids. The relationship between the synthesis ability of polyunsaturated fatty acids and the high content of polyunsaturated fatty acids in seawater was further confirmed.
【作者单位】：上海海洋大学农业部淡水水产种质资源重点实验室;
【基金】：国家自然科学基金(31472287) 水产动物遗传育种中心上海市协同创新中心(ZF1206)共同资助
【分类号】：S917.4

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