基于RNA-Seq数据筛选的银叶真藓耐干相关基因表达模式研究
发布时间：2018-12-26 18:07
【摘要】：本文以前期获得的银叶真藓在干旱-复水过程中的转录组数据为基础,选择了9个在干旱-复水条件下基因表达差异明显的相关耐干响应基因,并利用荧光定量PCR技术研究其在干旱-复水、ABA诱导胁迫及盐胁迫下的表达模式。研究结果表明,RT-q PCR实验获得的9个基因在干旱-复水下的表达趋势与RNA-Seq基因表达谱中基因表达规律基本一致,因此较好地验证了RNA-Seq数据的可靠性。在干旱-复水过程中,9个基因均不同程度受到干旱和复水诱导表达,但复水阶段基因诱导表达更为强烈,且大多数基因表达量在复水2 h达到高峰,如ERF、Dehydrin(DHN)和HSF3基因等。在ABA和盐胁迫处理下,9个耐干相关基因中有8个同时受ABA和盐诱导上调表达,其中ERF、DHN和LEA基因受ABA和盐胁迫上调表达最为显著。综上,ERF、DHN和LEA等基因同时受干旱-复水,ABA和高盐强烈诱导表达,可作为将来重点研究的抗逆基因。
[Abstract]:Based on the transcriptional data obtained in the early stage of drought and rehydration, nine genes related to dry tolerance were selected, which showed significant difference in gene expression under the condition of drought and rehydration. Fluorescence quantitative PCR technique was used to study its expression pattern under drought rehydration ABA induced stress and salt stress. The results show that the expression trend of 9 genes obtained in RT-q PCR experiment is basically consistent with that in RNA-Seq gene expression profile. Therefore, the reliability of RNA-Seq data is well verified. During the course of drought and rehydration, the nine genes were induced to express by drought and rehydration to varying degrees, but the induced expression was stronger in the rehydration stage, and the expression of most genes reached its peak at 2 h after rehydration, such as ERF,. Dehydrin (DHN) and HSF3 genes. Under ABA and salt stress, 8 of the 9 dry-tolerant genes were up-regulated by ABA and salt-induced, and ERF,DHN and LEA genes were most significantly up-regulated by ABA and salt stress. In conclusion, genes such as ERF,DHN and LEA were strongly induced by drought rehydration, ABA and high salt, and could be used as stress resistant genes in the future.
【作者单位】： 中国科学院新疆生态与地理研究所中国科学院干旱区生物地理与生物资源重点实验室;中国科学院大学;香港中文大学农业生物技术国家重点实验室;
【基金】：新疆维吾尔自治区自然科学基金面上项目(2015211A047)~~
【分类号】：Q943.2
本文编号：2392492
[Abstract]:Based on the transcriptional data obtained in the early stage of drought and rehydration, nine genes related to dry tolerance were selected, which showed significant difference in gene expression under the condition of drought and rehydration. Fluorescence quantitative PCR technique was used to study its expression pattern under drought rehydration ABA induced stress and salt stress. The results show that the expression trend of 9 genes obtained in RT-q PCR experiment is basically consistent with that in RNA-Seq gene expression profile. Therefore, the reliability of RNA-Seq data is well verified. During the course of drought and rehydration, the nine genes were induced to express by drought and rehydration to varying degrees, but the induced expression was stronger in the rehydration stage, and the expression of most genes reached its peak at 2 h after rehydration, such as ERF,. Dehydrin (DHN) and HSF3 genes. Under ABA and salt stress, 8 of the 9 dry-tolerant genes were up-regulated by ABA and salt-induced, and ERF,DHN and LEA genes were most significantly up-regulated by ABA and salt stress. In conclusion, genes such as ERF,DHN and LEA were strongly induced by drought rehydration, ABA and high salt, and could be used as stress resistant genes in the future.
【作者单位】： 中国科学院新疆生态与地理研究所中国科学院干旱区生物地理与生物资源重点实验室;中国科学院大学;香港中文大学农业生物技术国家重点实验室;
【基金】：新疆维吾尔自治区自然科学基金面上项目(2015211A047)~~
【分类号】：Q943.2
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