盐胁迫对南方泡桐基因表达的影响

发布时间：2019-01-01 10:48

【摘要】：为阐明泡桐多倍体的耐盐分子机制,以南方泡桐二倍体及对应的四倍体为试验材料,以白花泡桐基因组为参考序列,利用RNA-Seq测序技术,研究了盐处理前后南方泡桐四倍体和对应二倍体的基因表达变化。通过比对分析,共鉴定出与盐胁迫相关的差异表达基因2 940个。对这些差异基因进行GO功能分类,结果显示差异基因共参与了39个分类,集中在细胞、结合、催化等分类功能区的数量最多。KEGG代谢通路分析发现,这些差异表达基因主要参与了Plant hormone signal transduction,Carbon metabolism,Biosynthesis of amino acid等,其中编码MYB、WRKY、bZIP、ATPase等的基因差异表达显著。表明这些基因可能是潜在的盐胁迫响应基因。采用qRTPCR技术验证了随机挑选的9个差异表达基因,结果与转录组测序数据趋势一致。
[Abstract]:In order to elucidate the molecular mechanism of salt tolerance of Paulownia paulownia polyploid, the diploid and tetraploid of Paulownia paulownia were used as experimental materials, the genome of Paulownia paulownia was used as reference sequence, and the RNA-Seq sequencing technique was used. The changes of gene expression of paulownia tetraploid and diploid were studied before and after salt treatment. A total of 2940 differentially expressed genes related to salt stress were identified by comparative analysis. The GO functional classification of these differentially expressed genes showed that the differentially expressed genes were involved in 39 classifications, mainly in cell, binding, catalytic and other functional regions. KEGG metabolic pathway analysis showed that, These differentially expressed genes were mainly involved in Plant hormone signal transduction,Carbon metabolism,Biosynthesis of amino acid et al. Among them, the differentially expressed genes encoded MYB,WRKY,bZIP,ATPase et al. These genes may be potential salt stress response genes. Nine differentially expressed genes were randomly selected by qRTPCR. The results were consistent with the trend of transcriptome sequencing data.
【作者单位】：河南农业大学泡桐研究所;
【基金】：国家公益性林业行业科研专项(201004002)
【分类号】：S792.43

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