黄芪转录组基因模型预测

发布时间：2019-01-02 09:58

【摘要】：目的:黄芪作为传统补气中药具有悠久历史,从黄芪中已经提取出多种有效成分。目前黄芪的基因组还未进行测序,因此基于转录组数据预测基因模型,将有助于黄芪在医药领域的广泛应用。方法:本文基于黄芪转录组数据以及已有的亲缘关系相近物种全基因组序列,对黄芪转录组的unigene在基因组中的情况进行预测分析。结果:预测结果表明,黄芪和大豆Glycine max、蒺藜苜蓿Medicago truncatula、百脉根Lotus japonicus 3种植物有较近的亲缘关系,和蒺藜苜蓿比对上的基因数目是2 039条,碱基相似度是90.14%;和大豆比对上的基因数是1 948条,碱基相似度是90.40%;和百脉根比对上的基因数是1 003条,碱基相似度是89.77%。结论:黄芪与3种植物比对上的基因在3种植物的各染色体上,以及染色体外基因组上均有分布。
[Abstract]:Objective: Astragalus membranaceus has a long history as a traditional Chinese medicine for tonifying qi, and many active components have been extracted from Astragalus membranaceus. At present, the genome of Astragalus membranaceus has not been sequenced, so predicting gene model based on transcriptome data will be helpful to the wide application of Astragalus in medicine field. Methods: based on the transcriptional data of Astragalus membranaceus (Astragalus membranaceus) and the whole genome sequence of related species, the unigene of Astragalus membranaceus transcriptiongroup in genome was predicted and analyzed. Results: the predicted results showed that there was a close relationship between Astragalus membranaceus and Soybean Glycine max, Tribulus terrestris Medicago truncatula, Alfalfa Lotus japonicus, the number of genes compared with Tribulus terrestris alfalfa was 2 039, and the similarity of base was 90.14%. The number of genes compared with soybean is 1 948, the similarity of base is 90.40, and the number of genes compared with 100 veins is 1 003, and the similarity of base is 89.77. Conclusion: the genes in comparison between Astragalus membranaceus and three species of plants are distributed on the chromosomes of the three species and on the genome outside the chromosomes.
【作者单位】：黑龙江中医药大学药学院;
【基金】：黑龙江省杰出青年基金(JC201101) 国家自然科学基金(81274010) 黑龙江中医药大学“优秀创新人才支持计划”(2012001) 哈尔滨市优秀学科带头人基金(2014RFXXJ122) 黑龙江省教育厅科学技术研究项目(12541743)
【分类号】：S567.239

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