甜瓜海藻糖-6-磷酸合成酶基因鉴定及表达

发布时间：2019-01-03 12:08

【摘要】：为了明确甜瓜海藻糖-6-磷酸合成酶基因(CmTPS)家族信息及对逆境信号的响应,该研究采用生物信息学方法,通过拟南芥TPS家族基因与甜瓜基因组数据库比对,从甜瓜基因组中共鉴定出7个海藻糖-6-磷酸合成酶基因,按照其在染色体上的位置分别命名为CmTPS1~7。系统进化分析结果显示,CmTPS4和CmTPS7为第1类,二者均含有16个内含子,推测其编码产物均具有海藻糖-6-磷酸合成酶(TPS)活性;其余5个CmTPS基因归为第2类,分别含有2~4个内含子;在这7个甜瓜TPS中,除CmTPS3只有TPS结构域外,其余CmTPS都含有TPS、TPP及UDP-forming结构域;蛋白序列比对结果显示,甜瓜TPS家族各成员间相似性较低(15.90%~57.31%);亚细胞定位预测表明,CmTPS1、CmTPS2和CmTPS6定位在细胞核内,其余4个CmTPS定位在细胞质内。qRTPCR表达分析表明,低温胁迫下甜瓜叶片可能以CmTPS4为主要的TPS编码基因;CmTPS基因家族对盐胁迫较为敏感,同时在ABA信号传递中起调控作用。这为进一步研究甜瓜TPS基因家族奠定了基础。
[Abstract]:In order to identify the (CmTPS) family information of trehalose-6-phosphate synthase gene and its response to stress signals in muskmelon, the bioinformatics method was used to compare Arabidopsis TPS family gene with melon genomic database. Seven trehalose-6-phosphate synthase genes were identified from muskmelon genome and named CmTPS1~7. according to their position on chromosome. Phylogenetic analysis showed that CmTPS4 and CmTPS7 belonged to the first category, both of them contained 16 introns. It was inferred that the encoding products of them all had trehalose 6-phosphate synthase (TPS) activity. The other 5 CmTPS genes were classified as class 2, containing 2 ~ 4 introns respectively. In the 7 muskmelon TPS, except CmTPS3 with the TPS domain, the other CmTPS contained TPS,TPP and UDP-forming domains. The results of protein sequence alignment showed that the similarity among the members of muskmelon TPS family was lower (15.9057.31%). Subcellular localization prediction showed that CmTPS1,CmTPS2 and CmTPS6 were located in the nucleus and the other four CmTPS were located in the cytoplasm. QRTPCR expression analysis showed that CmTPS4 might be the main TPS coding gene in melon leaves under low temperature stress. CmTPS gene family is sensitive to salt stress and plays a regulatory role in ABA signaling. This lays a foundation for further study of TPS gene family in muskmelon.
【作者单位】：西北农林科技大学园艺学院;文山州农业科学院;
【基金】：国家现代农业产业技术体系建设专项(CARS-26-18)
【分类号】：Q943.2;S652

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