辣椒紫色酸性磷酸酶基因家族的鉴定与表达特征

发布时间：2019-01-06 11:41

【摘要】：紫色酸性磷酸酶(purple acid phosphatases,PAPs)属于金属磷酸酯酶家族,参与了植物大量的生理反应,特别是磷素的吸收与利用。本研究利用已经发表的辣椒基因组数据,采用同源比对方法对辣椒PAP基因家族进行鉴定与表达分析。通过鉴定发现辣椒基因组中至少含有25个PAP基因成员,氨基酸序列长度在264~639 aa,具有1~12个内含子,不均匀地分布在12条染色体上,仅发现1对基因以串联重复的形式存在。系统发育关系表明,辣椒、拟南芥和水稻PAP基因家族成员可以分为3个家族,8个亚家族,每个家族成员的数目是变异的,而且存在6对直系同源基因和20对旁系同源基因,这表明PAP基因家族的存在早于辣椒、拟南芥和水稻的分化。基于RNA-seq数据库和q RT-PCR方法,对辣椒PAP基因的组织表达及果实发育的不同时期进行分析,结果表明PAP家族基因具有组织表达特异性和发育时期表达特异性。
[Abstract]:Purple acid phosphatase (purple acid phosphatases,PAPs) belongs to the family of metal phosphatase, which is involved in a large number of physiological responses of plants, especially the absorption and utilization of phosphorus. In this study, the PAP gene family of capsicum was identified and expressed by using the published genomic data of capsicum (Capsicum capsicum). It was found that there were at least 25 members of PAP gene in the genome of capsicum. The amino acid sequence was 264n639 aa, with 1 ~ 12 introns, which was unevenly distributed on 12 chromosomes. Only one pair of genes was found to exist in the form of tandem repeats. Phylogenetic relationships showed that the members of PAP gene family of pepper, Arabidopsis thaliana and rice could be divided into 3 families, 8 subfamilies. The number of members of each family was variable, and there were 6 pairs of lineal homologous genes and 20 pairs of sequentially homologous genes. This indicated that the PAP gene family existed earlier than the differentiation of pepper, Arabidopsis and rice. Based on RNA-seq database and Q RT-PCR method, the tissue expression of PAP gene and fruit development of pepper were analyzed. The results showed that PAP family gene had tissue expression specificity and developmental stage expression specificity.
【作者单位】：苏州农业职业技术学院;浙江省植物有害生物防控重点实验室—省部共建国家重点实验室培育基地浙江省农业科学院蔬菜研究所;衢州市农业科学研究院;江苏沿海地区农业科学研究所;
【基金】：江苏省自然科学基金青年基金项目(BK20140277) 苏州市科技发展计划项目应用基础研究(SYN201418) 苏州农业职业技术学院青年教师科研能力提升计划资助项目(PPN201308) 浙江省自然科学基金(LQ15C150002) 国家自然科学基金(31501749);国家自然科学基金青年人才培养计划(2015R23R08E07,2015R23R08E09) 国家重点实验室开放项目(2010DS700124-KF1517)
【分类号】：S641.3

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