香蕉枯萎病菌4号生理小种cat1基因敲除与表型分析

发布时间：2019-01-06 17:37

【摘要】：前期蛋白质组学研究发现尖孢镰刀菌古巴专化型4号生理小种(Foc4)中的过氧化氢酶1(cat1)基因受到巴西蕉诱导上调表达,为研究cat1基因在Foc4侵染香蕉过程中的作用,利用同源重组方法敲除Foc4的cat1基因,并对获得的敲除突变体开展表型分析和致病性测定。结果表明:cat1基因缺失对Foc4的菌丝、孢子形态、菌落生长、抗渗透压胁迫和细胞壁选择性压力等没有影响;但引起菌株抵抗外源氧胁迫、细胞壁穿透能力、纤维素利用能力减弱和对巴西蕉的致病性减弱。显示cat1基因的产物通过清除香蕉分泌的活性氧在Foc4对香蕉的致病过程中起作用,并参与病原菌对寄主纤维素成分的代谢。此结果为进一步深入研究香蕉枯萎病菌的致病机理奠定基础。
[Abstract]:Prophase proteomics studies showed that catalase 1 (cat1) gene of Fusarium oxysporum Cuba-specific physiological race No. 4 (Foc4) was up-regulated by Canna brasiliensis, so as to study the role of cat1 gene in the process of Foc4 infection on banana. Homologous recombination method was used to knockout the cat1 gene of Foc4, and phenotypic analysis and pathogenicity of the knockout mutants were carried out. The results showed that cat1 gene deletion had no effect on hyphae, spore morphology, colony growth, osmotic stress resistance and cell wall selective pressure of Foc4. However, the resistance of the strain to exogenous oxygen stress, cell wall penetration, cellulose utilization and pathogenicity of Canna brasiliensis were weakened. The results showed that the products of cat1 gene played an important role in the pathogenesis of banana by scavenging the active oxygen species secreted from banana, and participated in the metabolism of host cellulose by pathogenic bacteria. The results laid a foundation for further study on the pathogenic mechanism of banana wilt pathogen.
【作者单位】：海南大学;中国热带农业科学院热带生物技术研究所;农业部热带作物生物技术重点实验室;
【基金】：海南耕地改良关键技术研究与示范专项(No.HNGDXF2015) 中国热带农业科学院热带生物技术研究所基本科研业务费(No.ITBB2015ZT02) 国家自然科学基金(No.31271997) 公益性行业农业科研专项(No.201503110)
【分类号】：S436.68

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