六味地黄丸对绝经期肾阴虚骨质疏松症的基因表达调控数据分析

发布时间：2019-01-09 12:50

【摘要】：目的利用生物信息学分析工具,从基因调控的角度阐述六味地黄丸治疗绝经期肾阴虚骨质疏松症的分子机制。方法运用生物信息学分析工具QOE、DAVID和STRING,对美国国家生物信息中心(National Center for Biotechnology Information,NCBI)中的GEO数据库中六味地黄丸治疗绝经期肾阴虚骨质疏松症的相关数据进行挖掘分析。结果生物信息学分析显示中药六味地黄丸在治疗绝经期肾阴虚骨质疏松症的过程中基因TNP1和MAP3K10的表达显著增加,而ZNF家族基因和XRN1、XPO1、NUP54等基因的表达显著下降。这些差异基因的功能富集和相关作用机理分析显示,下调XRN1基因和ZNF家族基因的表达可以降低其对雌激素表达的抑制;上调TNP1基因的表达促进雌激素与雌激素受体结合;上调MAP3K10基因的表达并下调XPO1基因和NUP54基因的表达能降低免疫应答。结论六味地黄丸对绝经期肾阴虚骨质疏松症的有益影响主要是通过促进雌激素表达及与其受体结合,并平衡免疫应答而促进骨组织的形成,从而达到改善骨质疏松。
[Abstract]:Objective to study the molecular mechanism of Liuwei Dihuang pills in treating osteoporosis due to deficiency of kidney yin in menopausal period by means of bioinformatics analysis. Methods using bioinformatics analysis tools QOE,DAVID and STRING, the data of Liuwei Dihuang pills in the GEO database of (National Center for Biotechnology Information,NCBI for the treatment of osteoporosis due to kidney yin deficiency in menopausal period were analyzed. Results Bioinformatics analysis showed that the expression of TNP1 and MAP3K10 increased significantly in the treatment of osteoporosis due to kidney yin deficiency, while the expression of ZNF family genes and XRN1,XPO1,NUP54 genes decreased significantly. The functional enrichment and related mechanism analysis of these differentially expressed genes showed that down-regulating the expression of XRN1 gene and ZNF family genes could reduce the inhibition of estrogen expression, and up-regulate the expression of TNP1 gene to promote the binding of estrogen and estrogen receptor. Upregulation of MAP3K10 gene expression and down-regulation of XPO1 gene and NUP54 gene decreased immune response. Conclusion the beneficial effect of Liuwei Dihuang pill on osteoporosis of kidney yin deficiency in menopausal period is to promote the formation of bone tissue by promoting the expression of estrogen and binding to its receptor and balancing the immune response so as to improve osteoporosis.
【作者单位】：南方医科大学基础医学院;
【分类号】：R285.6

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