葡萄VvCOL5基因克隆及在胚珠发育过程中的表达分析
发布时间：2019-01-09 16:05
【摘要】：以葡萄无核品种无核白和有核品种黑比诺为材料,克隆葡萄CONSTANS基因家族的1个成员并将其命名为VvCOL5基因,分析基因结构,进行染色体定位和系统发育树的构建,检测VvCOL5基因在无核和有核葡萄胚珠(幼种子)发育过程的表达变化。结果表明:VvCOL5基因cDNA长度为1 374bp,包含有2个外显子和1个内含子结构,并含有2个B-box结构域和1个CCT结构域,属于CO家族的第一类,在GenBank登录号为KX077245。在受精后胚珠(幼种子)发育过程中,VvCOL5基因在无核白与黑比诺中呈现出了相反的表达模式。其无核白受精后10~30d其表达缓慢上调,30~35d维持在较高水平,35~40d缓慢下降,后在40~45d又快速上调到较高水平;而在黑比诺中10~20d下降,在20~35d之间相对平稳,35~40d缓慢上升,最后40~45d时又下降到较低水平。无核白中VvCOL5基因在各时期的表达量均高于黑比诺,可见VvCOL5基因在无核和有核品种中表达差异较大,其与葡萄种子发育有一定关系。
[Abstract]:A member of grape CONSTANS gene family was cloned from seedless grape varieties denuclearized and Pinot Noir, named VvCOL5 gene, gene structure was analyzed, chromosome mapping and phylogenetic tree construction were carried out. To detect the expression of VvCOL5 gene during the development of seedless and nucleated grape ovule (young seeds). The results show that the length of cDNA of VvCOL5 gene is 1 374 BP, including 2 exons and 1 intron structure, 2 B-box domains and 1 CCT domain, which belong to the first class of CO family, and the GenBank accession number is KX077245.. During the development of ovules (young seeds) after fertilization, the VvCOL5 gene expressed in denuclearized and Pinot Noir. The expression increased slowly at 10 ~ 30 days after fertilization, maintained at a higher level at 30 ~ 35 days, decreased slowly at 35 ~ 40 days, and then increased to a higher level at 40 ~ 45 days after fertilization. In Pinot Noir, it decreased at 10 ~ 20 days, remained stable at 20 ~ 35 days, increased slowly at 35 ~ 40 days, and then decreased to a lower level at 40 ~ 45 days. The expression of VvCOL5 gene in denuclearized white was higher than that in Pinot noir at every stage. It was found that the expression of VvCOL5 gene in seedless and nucleated varieties was different and had a certain relationship with grape seed development.
【作者单位】： 西北农林科技大学园艺学院旱区作物逆境生物学国家重点实验室农业部西北地区园艺作物生物学与种质创制重点实验室;
【基金】：国家自然科学基金(31372023) 国家现代农业产业技术体系建设专项(CARS-30-yz-7) 杨凌示范区农业科技示范推广项目(2015-TS-10)
【分类号】：S663.1
，
本文编号：2405844
[Abstract]:A member of grape CONSTANS gene family was cloned from seedless grape varieties denuclearized and Pinot Noir, named VvCOL5 gene, gene structure was analyzed, chromosome mapping and phylogenetic tree construction were carried out. To detect the expression of VvCOL5 gene during the development of seedless and nucleated grape ovule (young seeds). The results show that the length of cDNA of VvCOL5 gene is 1 374 BP, including 2 exons and 1 intron structure, 2 B-box domains and 1 CCT domain, which belong to the first class of CO family, and the GenBank accession number is KX077245.. During the development of ovules (young seeds) after fertilization, the VvCOL5 gene expressed in denuclearized and Pinot Noir. The expression increased slowly at 10 ~ 30 days after fertilization, maintained at a higher level at 30 ~ 35 days, decreased slowly at 35 ~ 40 days, and then increased to a higher level at 40 ~ 45 days after fertilization. In Pinot Noir, it decreased at 10 ~ 20 days, remained stable at 20 ~ 35 days, increased slowly at 35 ~ 40 days, and then decreased to a lower level at 40 ~ 45 days. The expression of VvCOL5 gene in denuclearized white was higher than that in Pinot noir at every stage. It was found that the expression of VvCOL5 gene in seedless and nucleated varieties was different and had a certain relationship with grape seed development.
【作者单位】： 西北农林科技大学园艺学院旱区作物逆境生物学国家重点实验室农业部西北地区园艺作物生物学与种质创制重点实验室;
【基金】：国家自然科学基金(31372023) 国家现代农业产业技术体系建设专项(CARS-30-yz-7) 杨凌示范区农业科技示范推广项目(2015-TS-10)
【分类号】：S663.1
，
本文编号：2405844
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