姜黄素对人骨肉瘤细胞的生长抑制及对MAPK基因的调控作用研究

发布时间：2019-01-15 23:27

【摘要】：目的:研究姜黄素人成骨肉瘤细胞MG-63的生长抑制作用,并检测对骨肉瘤细胞中MAPK基因表达的调控作用,初步明确姜黄素对骨肉瘤细胞的作用机制。方法:以人骨肉瘤细胞株MG-63为研究对象,采用不同剂量姜黄素处理。MTT法检测5,10,15,20,25,30,35,40,50μmol/L姜黄素作用24,48,72 h对细胞的毒性作用;Transwell小室侵袭实验法检测5,10,15μmol/L姜黄素对细胞的侵袭能力,黏附实验法检测对细胞的黏附能力;Western blot法检测5,10,15μmol/L姜黄素作用后MAPK蛋白表达水平的影响,RT-PCR法检测对细胞中MAPK基因m RNA转录水平的影响。结果:姜黄素浓度15μmol/L,作用时间24 h时,对细胞的生长抑制呈时间和剂量依懒性(P0.001),当姜黄素浓度≤15μmol/L,作用时间为24 h时,对细胞无明显毒性作用,细胞存活率85%;细胞侵袭能力和黏附能力随着姜黄素浓度的增加而逐渐降低,以15μmol/L姜黄素浓度处理效果最明显(P0.05);姜黄素可明显抑制MAPK基因m RNA转录水平,其抑制作用与剂量呈高度依懒性(P0.05)。结论:姜黄素可通过抑制MAPK信号通路的活化及此信号通路靶基因MAPK的表达降低人骨肉瘤细胞株MG-63的侵袭性。
[Abstract]:Aim: to investigate the inhibitory effect of curcumin on the growth of human osteosarcoma cell line MG-63, and to investigate the effect of curcumin on the expression of MAPK gene in osteosarcoma cells, and to clarify the mechanism of curcumin on osteosarcoma cells. Methods: human osteosarcoma cell line MG-63 was treated with different doses of curcumin. The cytotoxicity of curcumin on human osteosarcoma cell line (MG-63) was detected by MTT method. The invasiveness of curcumin to cells was detected by Transwell chamber invasion assay, and the adhesion ability of curcumin to cells was detected by adhesion assay. Western blot assay was used to detect the effect of curcumin on the expression of MAPK protein, and RT-PCR assay was used to detect the transcription level of MAPK gene m RNA. Results: when the concentration of curcumin was 15 渭 mol/L, for 24 h, the inhibition of cell growth was time-dependent and dose-dependent (P0.001). When the concentration of curcumin was 鈮，

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