不同葡萄糖转运途径相关基因的过表达对菌株葡萄糖代谢及产物合成的影响
发布时间：2019-01-19 18:35
【摘要】：磷酸烯醇式丙酮酸-葡萄糖磷酸转移酶系统(phosphoenolpyruvate-dependent glucose phosphotransferase system,PTS~(Glc))和不依赖于PTS~(Glc)系统葡萄糖转运系统在葡萄糖转运和磷酸化过程中起重要作用。该文通过在葡萄糖代谢缓慢的L-赖氨酸产生菌Corynebacterium glutamicum(C.glutamicum)ZL-8中过表达肌醇透性酶基因(iol T1)、葡萄糖激酶基因(ppgk)和EIIGlc基因(pts G),研究葡萄糖转运途径相关基因对葡萄糖代谢速率、L-赖氨酸及副产物合成的影响。与出发菌株C.glutamicum ZL-8相比,重组菌C.glutamicum ZL-8/p ECXK-99E-ppgkiol T1(葡萄糖激酶酶活力提高了768%)和C.glutamicum ZL-8/p DXW-8-pts G(PTS~(Glc)系统酶活力提高了62.7%)葡萄糖消耗速率分别提高了16.7%和27.3%,L-赖氨酸产量分别提高了24.7%和16.4%。此外,有机酸和氨基酸分析结果表明,过表达不同葡萄糖转运途径基因对有机酸(丙酮酸、乳酸和乙酸)和副产物氨基酸(丙氨酸、缬氨酸、天冬氨酸)具有不同的影响。
[Abstract]:Phosphoenolpyruvate-glucose phosphotransferase system (phosphoenolpyruvate-dependent glucose phosphotransferase system,PTS~ (Glc) and PTS~ (Glc) independent glucose transport system) plays an important role in the process of glucose transport and phosphorylation. In this paper, inositol permeability enzyme gene (iol T1), glucokinase gene (ppgk) and EIIGlc gene (pts G), were overexpressed in ZL-8 of L- lysine producing bacteria (Corynebacterium glutamicum (C.glutamicum), where glucose metabolism is slow. To study the effects of glucose transporter related genes on glucose metabolism rate, L-lysine and by-product synthesis. Compared with the original strain C.glutamicum ZL-8, Glucose consumption of recombinant bacteria C.glutamicum ZL-8/p ECXK-99E-ppgkiol T1 (increased by 76.8%) and C.glutamicum ZL-8/p DXW-8-pts G (PTS~ (Glc) system increased by 62.7%) The rate increased by 16.7% and 27.3%, respectively. The yield of L-lysine increased by 24. 7% and 16. 4%, respectively. In addition, the analysis of organic acids and amino acids showed that overexpression of different glucose transporter genes had different effects on organic acids (pyruvate, lactic acid and acetic acid) and by-product amino acids (alanine, valine, aspartic acid).
【作者单位】： 江南大学生物工程学院工业生物技术教育部重点实验室;
【基金】：江苏省自然科学基金青年基金(BK20150149)
【分类号】：TQ922.3
，
本文编号：2411638
[Abstract]:Phosphoenolpyruvate-glucose phosphotransferase system (phosphoenolpyruvate-dependent glucose phosphotransferase system,PTS~ (Glc) and PTS~ (Glc) independent glucose transport system) plays an important role in the process of glucose transport and phosphorylation. In this paper, inositol permeability enzyme gene (iol T1), glucokinase gene (ppgk) and EIIGlc gene (pts G), were overexpressed in ZL-8 of L- lysine producing bacteria (Corynebacterium glutamicum (C.glutamicum), where glucose metabolism is slow. To study the effects of glucose transporter related genes on glucose metabolism rate, L-lysine and by-product synthesis. Compared with the original strain C.glutamicum ZL-8, Glucose consumption of recombinant bacteria C.glutamicum ZL-8/p ECXK-99E-ppgkiol T1 (increased by 76.8%) and C.glutamicum ZL-8/p DXW-8-pts G (PTS~ (Glc) system increased by 62.7%) The rate increased by 16.7% and 27.3%, respectively. The yield of L-lysine increased by 24. 7% and 16. 4%, respectively. In addition, the analysis of organic acids and amino acids showed that overexpression of different glucose transporter genes had different effects on organic acids (pyruvate, lactic acid and acetic acid) and by-product amino acids (alanine, valine, aspartic acid).
【作者单位】： 江南大学生物工程学院工业生物技术教育部重点实验室;
【基金】：江苏省自然科学基金青年基金(BK20150149)
【分类号】：TQ922.3
，
本文编号：2411638
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