微量元素对金银花绿原酸合成关键酶基因LjHCT和LjC3H1表达的影响研究

发布时间：2019-01-22 17:23

【摘要】：绿原酸是金银花(Lonicera japonica Thunb.)入药的主要化学成分,如何稳定和提高绿原酸的含量是近年来金银花研究的热点。本研究通过对金银花分别根施Fe、B和Mo 3种微量元素,比较处理前、后金银花中微量元素与绿原酸含量的变化,并定量分析3种微量元素对金银花绿原酸合成关键酶基因LjHCT和LjC3H1表达的影响。研究结果显示,高浓度的Fe处理对LjHCT基因的表达有明显的抑制作用,而中、低浓度的Fe处理可以促进LjHCT基因的表达;随着B和Mo元素浓度的提高,LjHCT基因的表达量也逐渐增加。低浓度的Fe处理可以促进LjC3H1基因的表达,而高浓度的Fe处理对LjC3H1基因的表达具有抑制作用;B元素对LjC3H1基因表达无显著影响,而高浓度的Mo处理可以促进LjC3H1基因的表达。根施中、低浓度的Fe元素,中、高浓度的Mo和B元素后金银花绿原酸含量显著增加;而根施高浓度的Fe元素后金银花中绿原酸含量显著减少。研究结果表明微量元素Fe、B和Mo可通过调节绿原酸生物合成关键酶基因的表达从而有效促进绿原酸的形成和积累。本研究为人工定向调控金银花绿原酸含量、开发人工栽培金银花专用微量元素肥料提供了理论依据。
[Abstract]:Lv Yuan Acid is (Lonicera japonica Thunb.) of Flos Lonicerae The main chemical constituents of Chinese medicine, how to stabilize and improve the content of Lv Yuan acid are the hot spot of honeysuckle research in recent years. In this study, Fe,B and Mo were applied to the root of honeysuckle, and the contents of trace elements and Lv Yuan in honeysuckle were compared before and after treatment. The effects of three trace elements on the expression of LjHCT and LjC3H1 genes of Lv Yuan acid biosynthesis in Flos Lonicerae were analyzed quantitatively. The results showed that the high concentration of Fe could inhibit the expression of LjHCT gene, while the low concentration of Fe could promote the expression of LjHCT gene, and the expression of LjHCT gene increased with the increase of the concentration of B and Mo elements. Low concentration of Fe treatment could promote the expression of LjC3H1 gene, but high concentration of Fe treatment could inhibit the expression of LjC3H1 gene, B element had no significant effect on LjC3H1 gene expression, but high concentration of Mo treatment could promote the expression of LjC3H1 gene. In root application, the content of Lv Yuan in honeysuckle increased significantly with low concentration of Mo and B element, but decreased with root application of high concentration of Fe element. The results showed that trace element Fe,B and Mo could effectively promote the formation and accumulation of Lv Yuan acid by regulating the expression of the key enzyme genes of Lv Yuan biosynthesis. This study provides a theoretical basis for the direct regulation of Lv Yuan acid content in honeysuckle flower and the development of special trace element fertilizer for artificial cultivation of honeysuckle.
【作者单位】：凯里学院化学与材料工程学院;怀化学院生物与食品工程学院;
【基金】：贵州省科技计划厅联合基金项目(黔科合LH字[2014]7219) 贵州省科学技术基金项目(黔科合J字[2015]2131) 贵州省教育厅重点项目(黔教合KY字[2014]281) 湖南省科技计划重点项目(2013FJ4324)~~
【分类号】：Q943.2;S567.79

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