哈蟆油蛋白对骨质疏松预防及ALP,Osteocalcin,Runx-2基因表达的调节作用

发布时间：2019-01-24 10:09

【摘要】：目的:观察哈蟆油蛋白(ROP)的抗骨质疏松作用,并探讨其对骨生长相关基因的调节作用。方法:采用去势法建立大鼠骨质疏松模型,随机分为空白组,模型组,戊酸雌二醇组,ROP高、中、低剂量组(0.15,0.075,0.037 5 g·kg~(-1));利用X射线技术测量股骨和腰椎骨密度(BMD);利用骨骼强度仪检测股骨的最大载荷量;应用实时荧光定量PCR(Real-time PCR)技术检测骨生长相关基因的表达。结果:与空白组比较,模型组股骨、腰椎骨密度明显降低(P0.05,P0.01),碱性磷酸酶(alkaline phosphatase,ALP),Osteocalcin,Runx-2基因表达均显著下调(P0.01);与模型组比较,ROP高、中剂量组腰椎BMD均有显著增加(P0.01),ROP高剂量组ALP,Osteocalcin,Runx-2基因表达显著上调(P0.01)。结论:ROP能显著提高大鼠BMD和最大载荷量;ROP可能通过上调ALP,Osteocalcin,Runx-2基因的mRNA表达,促进成骨分化,调节骨代谢平衡,从而发挥防治骨质疏松的作用。
[Abstract]:Aim: to observe the anti-osteoporosis effect of Oviductus Ranae (ROP) and to investigate its regulatory effect on bone growth-related genes. Methods: the ovariectomized rat model of osteoporosis was established. The rats were randomly divided into blank group, model group, estradiol valerate group, high, middle and low doses of ROP (0. 15 0. 075 g kg~ (-1);). Bone mineral density (BMD);) of femur and lumbar vertebrae was measured by X-ray technique, and bone growth related gene expression was detected by real-time fluorescence quantitative PCR (Real-time PCR). Results: compared with the blank group, the bone mineral density (BMD) of femur and lumbar vertebrae in the model group decreased significantly (P0.01), the expression of alkaline phosphatase (alkaline phosphatase,ALP) and Osteocalcin,Runx-2 gene decreased significantly (P0.01). Compared with the model group, the expression of ALP,Osteocalcin,Runx-2 gene increased significantly in the middle dose group and the middle dose group (P0.01), ROP high dose group, P0.01). Conclusion: ROP can significantly increase BMD and maximum load of rats, ROP may promote osteogenic differentiation and regulate the balance of bone metabolism by up-regulating mRNA expression of ALP,Osteocalcin,Runx-2 gene, so as to play the role of prevention and treatment of osteoporosis.
【作者单位】：长春中医药大学;
【基金】：吉林省科技发展计划项目(20150309008YY) 吉林省中医药科技项目(2017002)
【分类号】：R285.5

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