IMPDH基因克

发布时间：2019-02-12 15:22

【摘要】：旨在获得牦牛IMPDH基因序列并进行生物信息学分析,同时分析其在卵巢组织中的时序表达谱,为进一步研究卵巢生长发育及排卵机制奠定基础。通过RT-PCR技术克隆牦牛的IMPDH基因,并运用生物信息学方法分析不同物种序列进化关系,利用荧光定量qRT-PCR技术检测IMPDH基因在不同发育时期牦牛卵巢组织中的表达情况。结果显示,克隆得到牦牛1 623bp的IMPDH基因cDNA序列,内含1 545bp开放阅读框序列,编码514个氨基酸残基。与野牦牛的相应序列具有很高的同源性(99.4%),表明IMPDH基因在进化过程中相对保守。实时定量RT-PCR检测结果显示IMPDH基因的表达随年龄的增加而呈现上升趋势。荧光定量qRT-PCR检测结果显示IMPDH基因在卵巢卵泡期表达最强,显著高于红体形成期。结果表明IMPDH参与牦牛卵巢组织的生长发育,并在卵巢活动中有明显的表达规律,表明IMPDH基因在牦牛繁殖周期具有重要作用,为进一步研究牦牛IMPDH基因功能奠定基础。
[Abstract]:The aim of this study was to obtain the sequence of yak IMPDH gene and to analyze the sequence of its expression in ovarian tissue by bioinformatics analysis, so as to lay a foundation for further study of ovarian growth and ovulation mechanism. The IMPDH gene of yak was cloned by RT-PCR, and the evolutionary relationship of different species was analyzed by bioinformatics. The expression of IMPDH gene in yak ovary was detected by fluorescence quantitative qRT-PCR technique. The results showed that the cDNA sequence of IMPDH gene of yak 1 623bp was cloned, which contained 1 545bp open reading frame sequence and encoded 514 amino acid residues. There was a high homology (99.4%) with the corresponding sequence of wild yak, indicating that the IMPDH gene was relatively conserved in the course of evolution. Real-time quantitative RT-PCR analysis showed that the expression of IMPDH gene increased with age. The results of fluorescence quantitative qRT-PCR showed that the expression of IMPDH gene was the strongest in follicular stage, and was significantly higher than that in erythropoiesis stage. The results showed that IMPDH was involved in the growth and development of yak ovary and had obvious expression rule in ovarian activity. The results showed that IMPDH gene played an important role in the reproductive cycle of yak, which laid a foundation for further study on the function of yak IMPDH gene.
【作者单位】：西南民族大学生命科学与技术学院;西南民族大学青藏高原研究院;
【基金】：四川省科技支撑计划(2014NZ0114) 西南民族大学中央高校基本科研业务费专项资金(2016NZYQN38)~~
【分类号】：Q78;S823.85

【相似文献】