金鱼草花青素糖基转移酶基因的克隆与表达特性分析
发布时间：2019-02-16 21:45
【摘要】：以白苏子(Perilla frutescens)花青素糖基转移酶基因为探针,通过电子克隆和RT-PCR的方法从金鱼草(Antirrhinum majus L.)叶片中克隆到花青素糖基转移酶基因(Am GT1)的全长c DNA,并对其表达特性进行分析。结果表明:Am GT1全长892 bp,编码277个氨基酸;进化分析表明Am GT1的氨基酸序列与白苏子的同源性最高为79%,与其他花青素糖基转移酶蛋白的同源性在42%~62%之间,表明Am GT1是从金鱼草中克隆的新的花青素糖基转移酶基因。实时定量RT-PCR分析表明:该基因在金鱼草叶片中的表达量最高,根中的表达量最低;该基因虽然在红色、粉色、黄色、白色花朵中均有表达,但在红色花朵中的表达量最高,且存在一个从紧蕾期到松蕾期的跃变。
[Abstract]:(Perilla frutescens) anthocyanidin glycosyltransferase was used as a probe, and (Antirrhinum majus L.) was obtained by electronic cloning and RT-PCR. The full-length c DNA, of anthocyanin glycosyltransferase gene (Am GT1) was cloned from leaves and its expression characteristics were analyzed. The results showed that the total length of Am GT1 was 892 bp, encoding 277 amino acids. Evolutionary analysis showed that the amino acid sequence of Am GT1 had the highest homology with Perilla perilla, and the homology with other anthocyanin glycosyltransferase proteins was between 42% and 62%. The results indicate that Am GT1 is a new anthocyanin glycosyltransferase gene cloned from Goldfish. Real-time quantitative RT-PCR analysis showed that the expression of the gene was the highest in the leaves and the lowest in the roots. Although the gene was expressed in red, pink, yellow and white flowers, it had the highest expression in red flowers, and there was a jump from tight bud stage to pine bud stage.
【作者单位】： 漳州城市职业学院园林园艺系;贵州省烟草科学研究院/烟草行业分子遗传重点实验室;
【基金】：中国烟草总公司贵州省公司项目(No.201501)
【分类号】：S681.9
[Abstract]:(Perilla frutescens) anthocyanidin glycosyltransferase was used as a probe, and (Antirrhinum majus L.) was obtained by electronic cloning and RT-PCR. The full-length c DNA, of anthocyanin glycosyltransferase gene (Am GT1) was cloned from leaves and its expression characteristics were analyzed. The results showed that the total length of Am GT1 was 892 bp, encoding 277 amino acids. Evolutionary analysis showed that the amino acid sequence of Am GT1 had the highest homology with Perilla perilla, and the homology with other anthocyanin glycosyltransferase proteins was between 42% and 62%. The results indicate that Am GT1 is a new anthocyanin glycosyltransferase gene cloned from Goldfish. Real-time quantitative RT-PCR analysis showed that the expression of the gene was the highest in the leaves and the lowest in the roots. Although the gene was expressed in red, pink, yellow and white flowers, it had the highest expression in red flowers, and there was a jump from tight bud stage to pine bud stage.
【作者单位】： 漳州城市职业学院园林园艺系;贵州省烟草科学研究院/烟草行业分子遗传重点实验室;
【基金】：中国烟草总公司贵州省公司项目(No.201501)
【分类号】：S681.9
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