干旱胁迫对蒲公英渗透调节物质、酶保护系统及质膜水孔蛋白PIP2-3基因表达的影响

发布时间：2019-02-23 21:36

【摘要】：为了揭示蒲公英在干旱胁迫下渗透调节和酶保护系统的作用机理以及质膜水孔蛋白PIP2-3基因的表达特征,采用盆栽控水试验,研究了不同干旱胁迫RWC=(90±5)%、RWC=(75±5)%、RWC=(50±5)%下蒲公英渗透调节物质、酶保护系统以及质膜水孔蛋白基因的相关表达。结果表明:随着干旱胁迫的加重,蒲公英叶片中的可溶性糖、可溶性蛋白、游离脯氨酸的含量显著增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性显著提高,在轻度胁迫(MS)中呈持续上升的趋势,重度胁迫(SS)中则呈现先升高后下降的趋势(可溶性糖除外);质膜水孔蛋白PIP2-3基因的相对表达量上调,轻度胁迫(MS)显著高于重度胁迫(SS)。复水7 d后,渗透调节物质的积累以及SOD的含量下降,但仍显著高于CK,POD、CAT的含量以及质膜水孔蛋白PIP2-3基因的相对表达量恢复正常,说明在干旱胁迫条件下蒲公英通过增加渗透调节物质的积累,提高酶保护系统的活性来增强植株的抗逆性,上调质膜水孔蛋白基因的相对表达量来加强水分运输能力。
[Abstract]:In order to reveal the mechanism of osmotic regulation and enzyme protection system of dandelion under drought stress and the expression characteristics of plasma membrane aquaporin PIP2-3 gene, the RWC= (90 卤5)% and RWC= (75 卤5)% under different drought stress were studied by pot water control experiment. RWC= (50 卤5)%. The osmotic regulator, enzyme protective system and the expression of aquaporin gene in the plasma membrane of dandelion were studied. The results showed that the contents of soluble sugar, soluble protein and free proline in dandelion leaves increased significantly with the increase of drought stress, and the content of superoxide dismutase (SOD),) peroxidase (POD),) in the leaves of dandelion increased significantly. The activity of catalase (CAT) increased significantly, and the activity of catalase (CAT) increased significantly in mild stress (MS), while in severe stress (SS) increased first and then decreased (except soluble sugar). The relative expression of plasma membrane aquaporin PIP2-3 gene was up-regulated, and the level of (MS) under mild stress was significantly higher than that of (SS). Under severe stress. After 7 days of rehydration, the accumulation of osmotic regulators and the content of SOD decreased, but still significantly higher than the content of CK,POD,CAT and the relative expression of PIP2-3 gene of plasma membrane aquaporin returned to normal. The results showed that dandelion could enhance plant resistance by increasing the accumulation of osmotic regulatory substances, enhancing the activity of enzyme protection system, and increasing the relative expression of plasma membrane aquaporin gene to enhance the water transport ability under drought stress.
【作者单位】：四川农业大学园艺学院;
【基金】：四川省教育厅项目(10ZB044)
【分类号】：S567.239

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