大豆中介体亚基基因鉴定及表达特性分析
发布时间：2019-02-28 15:46
【摘要】：中介体复合物在真核生物蛋白编码基因和非编码RNA的转录中非常重要。为了分析大豆中介体亚基的序列特征和基因表达模式,应用BLASTp获取与拟南芥中介体亚基同源的蛋白序列,用Clustal Omega、MAFFT、MEGA等方法进行多序列联配和进化分析,同时应用Me V构建了基因的表达量热图。结果表明:共获得118个基因位点(Locus),186个转录本,在大豆20条染色体上均有分布。Med8的N端具有TBP结构域,Med31的C端具有脯氨酸富集区和假定的核定位信号,Med16的C端有C2-C2型锌指结构域(Zinc-finger motif)。栽培大豆与野生大豆的同源蛋白亲缘关系最近,进化分析符合物种进化规律。大豆不同中介体亚基基因的表达量差异很大,但同一基因在不同组织中的表达差异较小,仅有3个基因在根瘤中特异表达,1个基因在豆荚中特异表达。脱水处理后5个中介体亚基基因表达上调明显,Na Cl处理1和6 h后分别有2和3个中介体亚基基因表达显著上调。这为进一步分析中介体亚基在大豆发育和逆境胁迫中的分子功能奠定基础。
[Abstract]:Intermediate complexes are very important in the transcription of eukaryotic protein-encoded genes and non-coding RNA. In order to analyze the sequence characteristics and gene expression patterns of soybean intermediary subunits, BLASTp was used to obtain protein sequences homologous to Arabidopsis intermediary subunits, and multiple sequence association and evolutionary analysis were carried out by Clustal Omega,MAFFT,MEGA and other methods. At the same time, the heat map of gene expression was constructed by using Me V. The results showed that a total of 118 (Locus), transcripts were obtained and distributed on 20 chromosomes of soybean. The N-terminal of Med8 had TBP domain, and the C-terminal of Med31 had proline-rich region and assumed nuclear localization signal. The C terminal of Med16 has C2C2 zinc finger domain (Zinc-finger motif). The phylogenetic relationship of homologous proteins between cultivated soybean and wild soybean was recent, and the evolutionary analysis was in accordance with the evolution law of species. The expression of subunits of different mediators in soybean varied greatly, but the expression of the same gene in different tissues was small. Only 3 genes were specifically expressed in root nodules and 1 gene was specifically expressed in pods. The gene expression of 5 mediators subunits was up-regulated after, Na Cl treatment for 1 h and 6 h, respectively, and the gene expressions of 2 and 3 intermediary subunits were up-regulated after dehydration treatment. This provides a basis for further analysis of the molecular functions of intermediary subunits in soybean development and stress.
【作者单位】： 河南省农业科学院经济作物研究所;河南农业大学农学院;
【基金】：国家自然科学基金(31371652) 国家高技术研究发展计划“863计划”(2012AA101106)
【分类号】：S565.1
本文编号：2431936
[Abstract]:Intermediate complexes are very important in the transcription of eukaryotic protein-encoded genes and non-coding RNA. In order to analyze the sequence characteristics and gene expression patterns of soybean intermediary subunits, BLASTp was used to obtain protein sequences homologous to Arabidopsis intermediary subunits, and multiple sequence association and evolutionary analysis were carried out by Clustal Omega,MAFFT,MEGA and other methods. At the same time, the heat map of gene expression was constructed by using Me V. The results showed that a total of 118 (Locus), transcripts were obtained and distributed on 20 chromosomes of soybean. The N-terminal of Med8 had TBP domain, and the C-terminal of Med31 had proline-rich region and assumed nuclear localization signal. The C terminal of Med16 has C2C2 zinc finger domain (Zinc-finger motif). The phylogenetic relationship of homologous proteins between cultivated soybean and wild soybean was recent, and the evolutionary analysis was in accordance with the evolution law of species. The expression of subunits of different mediators in soybean varied greatly, but the expression of the same gene in different tissues was small. Only 3 genes were specifically expressed in root nodules and 1 gene was specifically expressed in pods. The gene expression of 5 mediators subunits was up-regulated after, Na Cl treatment for 1 h and 6 h, respectively, and the gene expressions of 2 and 3 intermediary subunits were up-regulated after dehydration treatment. This provides a basis for further analysis of the molecular functions of intermediary subunits in soybean development and stress.
【作者单位】： 河南省农业科学院经济作物研究所;河南农业大学农学院;
【基金】：国家自然科学基金(31371652) 国家高技术研究发展计划“863计划”(2012AA101106)
【分类号】：S565.1
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