碳酸氢钠对嗜水气单胞菌致病性及其主要毒力相关基因表达的影响

发布时间：2019-03-01 19:21

【摘要】：为探讨碳酸氢钠对嗜水气单胞菌毒力基因表达的影响,利用荧光定量PCR技术,以未添加碳酸氢钠的嗜水气单胞菌为对照组,添加0.024、0.048、0.072、0.096和0.120 mol/L碳酸氢钠的嗜水气单胞菌为实验组,对嗜水气单胞菌的3个主要毒力基因——溶血素基因(AHH-1)、气溶素基因(Aer A)和弹性蛋白酶基因(ahyB)进行相对表达分析。结果表明,5个实验组嗜水气单胞菌的AHH-1、AerA和ahyB表达量均显著低于对照组。且随着碳酸氢钠浓度的增加,基因表达呈逐渐下调趋势。人工感染结果表明,添加碳酸氢钠培养的嗜水气单胞菌对斑马鱼的半致死量明显比对照组高,而且随着碳酸氢钠浓度的增加,嗜水气单胞菌对斑马鱼的半致死量呈现逐渐加大的趋势,表明毒力逐渐减小。
[Abstract]:In order to investigate the effect of sodium bicarbonate on the expression of virulence genes of Aeromonas hydrophila, the non-sodium bicarbonate-added Aeromonas hydrophila was used as control group by fluorescence quantitative PCR (FQ-PCR). Aeromonas hydrophila added 0.024,0.048,0.072, 0.096 and 0.120 mol/L of sodium bicarbonate was used as the experimental group. The three main virulence genes of Aeromonas hydrophila-hemolysin gene (AHH-1) were tested. The relative expression of aerolysin gene (Aer A) and elastase gene (ahyB) were analyzed. The results showed that the expression levels of AHH-1,AerA and ahyB of Aeromonas hydrophila in 5 experimental groups were significantly lower than those in control group. And with the increase of the concentration of sodium bicarbonate, the gene expression was down-regulated gradually. The results of artificial infection showed that the semi-lethal rate of Aeromonas hydrophila cultured with sodium bicarbonate to zebrafish was significantly higher than that of control group, and with the increase of sodium bicarbonate concentration, The half lethal dose of Aeromonas hydrophila to zebrafish gradually increased, indicating that the virulence of Zebra fish decreased gradually.
【作者单位】：中国水产科学研究院珠江水产研究所农业部热带亚热带水产资源利用与养殖重点实验室;上海海洋大学水产与生命学院;贵州大学动物科学学院;
【基金】：国家科技支撑计划项目(2012BAD25B01) 广东省海洋渔业科技推广专项(B201400C01) 中国水产科学研究院基本科研业务费专项(2016HY-ZD0703) 国家现代农业产业技术体系建设专项(CARS-46-17)
【分类号】：S941.4

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