枯草芽孢杆菌HAS中TasA基因的克隆与分析
发布时间：2019-03-07 15:50
【摘要】：为弄清枯草芽孢杆菌HAS对甘蔗黑穗病菌的抑制作用机理,选取可对多种植物病原真菌有抑制作用的抗菌蛋白TasA基因进行克隆与分析。结果表明:在HAS菌株中含有TasA基因,克隆编码抗菌蛋白TasA的基因全长,序列分析其有786个碱基组成,该序列与GenBank上登录的TasA(AJ871386.1)序列同源性达99%,推测蛋白分子量大小大约28KD,其中第104、164、169、250、399、623、627位等7个碱基发生碱基转换或颠换,而且这些变异分别发生在密码子的第2、2、3、1、3、2、3位碱基上,引起多肽链氨基酸的错义突变。经氨基酸序列比对,同Bacillus subtilis subsp.subtilis str.168编码的TasA蛋白(NP_390342.1)在第150位和第209位上的氨基酸发生变化,由苏氨酸、天冬酰胺分别代替丙氨酸和谷氨酸。
[Abstract]:In order to find out the inhibitory mechanism of Bacillus subtilis HAS on the sugarcane smut, the anti-bacterial protein TasA gene, which can inhibit various plant pathogenic fungi, was selected to clone and analyze. The results showed that in the HAS strain, the gene of the TasA gene was cloned and the total length of the gene encoding the antibacterial protein TasA was cloned. The sequence analysis showed that the sequence was up to 99% and the molecular weight of the putative protein was about 28KD. In which 7 bases, such as 104,164,169,250,399,623,627, have base conversion or change, and these variations occur at the 2nd, 2nd, 3rd, 1st, 3rd, 2nd and 3rd bases of the codons, respectively, to cause the missense mutation of the amino acid of the polypeptide chain. The amino acids of the TasA protein (NP-390342.1) encoded by the Bacillus subtilis subsp.subtilis str.168 at the positions 150 and 209 were changed by the amino acid sequence, and the amino acids in the positions 150 and 209 were substituted for alanine and glutamic acid, respectively.
【作者单位】： 中国热带农业科学院热带生物技术研究所/甘蔗研究中心;海南医学院理学院;
【基金】：国家自然科学基金(面上项目)“甘蔗黑穗病拮抗菌株HAS抑菌机理研究”(31471555) 海南省自然科学基金项目“甘蔗黑穗病拮抗菌株HAS防病机制研究”(314120) 现代农业产业技术体系建设专项资金甘蔗植保岗位科学家经费(nycytx-24)
【分类号】：S476.1;S435.661
，
本文编号：2436242
[Abstract]:In order to find out the inhibitory mechanism of Bacillus subtilis HAS on the sugarcane smut, the anti-bacterial protein TasA gene, which can inhibit various plant pathogenic fungi, was selected to clone and analyze. The results showed that in the HAS strain, the gene of the TasA gene was cloned and the total length of the gene encoding the antibacterial protein TasA was cloned. The sequence analysis showed that the sequence was up to 99% and the molecular weight of the putative protein was about 28KD. In which 7 bases, such as 104,164,169,250,399,623,627, have base conversion or change, and these variations occur at the 2nd, 2nd, 3rd, 1st, 3rd, 2nd and 3rd bases of the codons, respectively, to cause the missense mutation of the amino acid of the polypeptide chain. The amino acids of the TasA protein (NP-390342.1) encoded by the Bacillus subtilis subsp.subtilis str.168 at the positions 150 and 209 were changed by the amino acid sequence, and the amino acids in the positions 150 and 209 were substituted for alanine and glutamic acid, respectively.
【作者单位】： 中国热带农业科学院热带生物技术研究所/甘蔗研究中心;海南医学院理学院;
【基金】：国家自然科学基金(面上项目)“甘蔗黑穗病拮抗菌株HAS抑菌机理研究”(31471555) 海南省自然科学基金项目“甘蔗黑穗病拮抗菌株HAS防病机制研究”(314120) 现代农业产业技术体系建设专项资金甘蔗植保岗位科学家经费(nycytx-24)
【分类号】：S476.1;S435.661
，
本文编号：2436242
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