红麻肌醇加氧酶基因的分离及表达分析
发布时间：2019-03-14 13:05
【摘要】：干旱是限制植物生长分布和影响作物产量形成的重要非生物因素之一,在响应干旱胁迫过程中植物细胞壁发挥着重要的调节作用,肌醇加氧酶是植物细胞壁合成的前体物质UDP-葡萄糖醛酸合成中的关键酶。为研究该基因在红麻(Hibiscus cannabinus L.)抗旱和耐盐过程中的作用,通过红麻转录组数据库筛选到肌醇加氧酶(myo-inositol oxygenase)基因的核心片段,利用染色体步移技术获得肌醇加氧酶基因的c DNA全长,命名为Hc MIOX;生物信息学分析表明,Hc MIOX基因编码序列长948 bp,编码315个氨基酸,相对分子量为31.9 ku,等电点为4.75;RT-PCR分析表明,Hc MIOX基因在红麻根、茎、叶中均有表达,根中表达量最高,该研究结果对分析该基因功能和了解红麻抗旱的分子机制具有重要意义。
[Abstract]:Drought is one of the important abiotic factors that limit plant growth distribution and crop yield formation, and plays an important role in the regulation of plant cell wall in response to drought stress. Inositol oxygenase is the key enzyme in the synthesis of UDP- glucuronic acid, the precursor of plant cell wall synthesis. In order to study the gene in kenaf (Hibiscus cannabinus L.) In the process of drought resistance and salt tolerance, the core fragment of inositol oxygenase (myo-inositol oxygenase) gene was screened by kenaf transcript database. The full length of inositol oxygenase gene c DNA, named Hc MIOX;, was obtained by chromosome step transfer technique. Bioinformatics analysis showed that the coding sequence of, Hc MIOX gene was 948 bp, long and encoded 315 amino acids, and the relative molecular weight was 31.9 ku, isoelectric point 4.75; RT-PCR analysis showed that, Hc MIOX gene was expressed in the roots, stems and leaves of kenaf, and the highest expression was found in the roots. The results of this study are of great significance for analyzing the function of the gene and understanding the molecular mechanism of drought resistance of kenaf.
【作者单位】： 上饶师范学院生命科学学院;中国农业科学院麻类研究所;
【基金】：江西省教育厅科学技术研究项目(编号:GJJ151050)) 中国农业科学院创新工程(编号:ASTIP-IBFC03) 上饶师范学院2014年博士科研启动基金(编号:001055)
【分类号】：Q943.2;S563.5
本文编号：2440007
[Abstract]:Drought is one of the important abiotic factors that limit plant growth distribution and crop yield formation, and plays an important role in the regulation of plant cell wall in response to drought stress. Inositol oxygenase is the key enzyme in the synthesis of UDP- glucuronic acid, the precursor of plant cell wall synthesis. In order to study the gene in kenaf (Hibiscus cannabinus L.) In the process of drought resistance and salt tolerance, the core fragment of inositol oxygenase (myo-inositol oxygenase) gene was screened by kenaf transcript database. The full length of inositol oxygenase gene c DNA, named Hc MIOX;, was obtained by chromosome step transfer technique. Bioinformatics analysis showed that the coding sequence of, Hc MIOX gene was 948 bp, long and encoded 315 amino acids, and the relative molecular weight was 31.9 ku, isoelectric point 4.75; RT-PCR analysis showed that, Hc MIOX gene was expressed in the roots, stems and leaves of kenaf, and the highest expression was found in the roots. The results of this study are of great significance for analyzing the function of the gene and understanding the molecular mechanism of drought resistance of kenaf.
【作者单位】： 上饶师范学院生命科学学院;中国农业科学院麻类研究所;
【基金】：江西省教育厅科学技术研究项目(编号:GJJ151050)) 中国农业科学院创新工程(编号:ASTIP-IBFC03) 上饶师范学院2014年博士科研启动基金(编号:001055)
【分类号】：Q943.2;S563.5
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