辣椒全基因组中CBL、CIPK基因家族的鉴定及特性分析

发布时间：2019-03-17 10:34

【摘要】：CBL、CIPK作为钙离子介导的信号转导通路上的重要蛋白在植物逆境应答和生长发育过程中发挥着重要的作用。本研究通过本地t BLASTn鉴定出辣椒中CBL、CIPK基因家族成员序列信息;运用MEGA程序构建系统进化树;利用GSDS程序对基因结构保守性进行分析、MEME程序进行基序分析以及UGENE、plant CARE软件对顺势作用元件进行了分析,显示9个辣椒CBL基因,21个辣椒CIPK基因分别分布于不同的染色体上,Ca CBL基因内含子个数保守为7~9个;Ca CIPK基因分为多内含子(11~14)和少内含子(0~1)两类。在辣椒CBL基因和CIPK基因的上游1 000 bp序列中存在多个应答不同逆境和植物激素的顺式作用元件且种类和数目各不相同。相互作用预测发现,辣椒CBL-CIPK可能存在多个相互作用通路。本研究可为进一步研究辣椒CBL、CIPK基因的功能以及辣椒中CBL-CIPK介导的Ca~(2+)信号转导通路提供有益参考。
[Abstract]:As an important protein in calcium-mediated signal transduction pathway, CBL,CIPK plays an important role in plant stress response and growth and development. In this study, the sequence information of CBL,CIPK gene family members in capsicum was identified by local t-BLASTn, and phylogenetic tree was constructed by MEGA program. GSDS program was used to analyze the conservatism of gene structure, MEME program was used for motif analysis and UGENE,plant CARE software was used for analysis of homeopathic elements. Nine CBL genes were found in pepper (Capsicum annuum L.). The number of introns of, Ca CBL gene in 21 capsicum peppers distributed on different chromosomes was conserved to 7? 9? Ca CIPK gene is divided into two types: polyintron (11 / 14) and few intron (0 / 1). There are several cis-acting elements that respond to different stresses and plant hormones in the upstream 1 000 bp sequence of CBL gene and CIPK gene in pepper, and the species and number of them are different. The interaction prediction showed that there might be multiple interaction pathways in pepper CBL-CIPK. This study may provide a useful reference for further study on the function of CBL,CIPK gene and CBL-CIPK-mediated Ca~ (2) signal transduction pathway in capsicum.
【作者单位】：东北农业大学园艺园林学院;东北农业大学生命科学学院;
【基金】：黑龙江省杰出青年基金(JC2015004) 东北农业大学校青年才俊计划(14QC11)共同资助
【分类号】：Q943.2;S641.3

【相似文献】