泄浊解毒方对溃疡性结肠炎大鼠结肠组织TLR2基因、蛋白表达的影响

发布时间：2019-04-02 12:01

【摘要】：目的:观察泄浊解毒方对溃疡性结肠炎(UC)大鼠肠黏膜上皮细胞(IECs)TLR2基因、蛋白表达的影响。方法:40只健康清洁级SD大鼠随机分5组:空白组,模型组,柳氮磺胺吡啶组,泄浊解毒方大、小剂量组,除正常组外采用2,4,6-三硝基苯磺酸(TNBS)/乙醇混合溶液灌肠复制UC大鼠模型,造模成功后,各治疗组大鼠灌服相应药物,正常组及模型组予0.9%氯化钠溶液灌胃。2周后处死全部大鼠,RT-PCR法检测TLR2基因表达,免疫组化法检测TLR2蛋白表达。结果:泄浊解毒方各剂量组大鼠TLR2基因、蛋白表达均明显低于模型组;泄浊解毒方大剂量组TLR2 mRNA、蛋白表达明显低于柳氮磺胺吡啶组及小剂量组。结论:泄浊解毒方对大鼠UC有良好的治疗作用,其作用机制可能与降低TLR2基因、蛋白表达有关。
[Abstract]:Aim: to observe the effect of Xiezhuangjiedu recipe on the expression of (IECs) TLR2 gene and protein in intestinal epithelial cells of (UC) rats with ulcerative colitis. Methods: 40 healthy clean grade SD rats were randomly divided into 5 groups: blank group, model group, sulfasalazine group, Xiezhuo jiedu prescription large and low dose group. The UC rat model was established by enema with 6-trinitrobenzene sulfonic acid (TNBS) / ethanol mixed solution. After successful establishment of the model, the rats in each treatment group were given the corresponding drugs, and the normal group and the model group were given 0.9% sodium chloride solution intragastrically. 2 weeks later, all the rats were killed. The expression of TLR2 gene was detected by RT-PCR method, and the expression of TLR2 protein was detected by immunohistochemical method. Results: the expression of TLR2 gene and protein in each dose group of Xiezhuangjiedu recipe was significantly lower than that in model group, while the expression of TLR2 mRNA, protein in large dose group of Xiezhuo jiedu recipe group was significantly lower than that in sulfasalazine group and low dose group. Conclusion: Xiezhuangjiedu recipe has a good therapeutic effect on UC in rats, and its mechanism may be related to the decrease of TLR2 gene and protein expression.
【作者单位】：河北省中医院;
【基金】：2014年河北省自然科学基金项目(No.H2014206462)~~
【分类号】：R285.5

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