高粱颖花开放前浆片茉莉酸信号基因表达变化

发布时间：2019-04-03 19:17

【摘要】：茉莉酸(JA)在植物生殖发育中发挥重要调控作用,尤其是促进禾本科植物的颖花开放。然而迄今有关高粱颖花开放削浆片内JA信号相关基因的表达变化模式与颖花开放时间的关系尚不清楚。以常规高粱种质"625R"为试材,采用RNA-seq技术分析高粱颖花开放前12 h和1 h的浆片转录组差异;并通过与数据库比对,分析高粱颖花开放前茉莉酸生物合成及信号转导途径相关基因的表达变化。结果表明,2个时期浆片组织中富集于茉莉酸生物合成及信号转导途径的差异基因有51个,且以花前12 h为对照,上调基因有41个,下调基因10个。茉莉酸生物合成途径中,差异基因主要涉及JA合成的上游调控步骤,而JA信号转导途径中则以JAZ蛋白响应基因数目最多、强度最大。研究结果为进一步阐明颖花开放进程中浆片吸水膨大的分子机制提供依据。
[Abstract]:Jasmonic acid (JA) plays an important role in the regulation of plant reproduction and development, especially in promoting the opening of glumes in gramineae. However, the relationship between the expression patterns of JA signal-related genes and the opening time of glumes in sorghum glumes is still unclear. Using the conventional sorghum germplasm "625R" as the test material, RNA-seq technique was used to analyze the difference of the plasmin transcripts at 12 h and 1 h before the flowering of the glumes in sorghum. The expression changes of jasmonic acid biosynthesis and signal transduction pathway related genes in sorghum glumes before opening were analyzed by comparing with the database. The results showed that there were 51 differential genes rich in jasmonic acid biosynthesis and signal transduction pathway in plasma tissue at two stages, and 41 genes were up-regulated and 10 genes down-regulated in 12 h before anthesis. In the jasmonic acid biosynthesis pathway, the differential genes are mainly involved in the upstream regulatory steps of JA synthesis, while in the JA signal transduction pathway, the number and intensity of JAZ protein response genes are the largest. The results provide a basis for further elucidating the molecular mechanism of swelling of plasmin during the opening process of glumes.
【作者单位】：江西农业大学作物生理生态与遗传育种教育部重点实验室;宜春学院生命科学与资源环境学院;
【基金】：国家自然科学基金项目(31360297) 江西省“赣鄱英才555工程”项目江西省科技支撑计划项目(20111BBF60009)~~
【分类号】：S514

【相似文献】