粗山羊草Dof转录因子家族基因的鉴定与分析

发布时间：2019-04-09 14:22

【摘要】：Dof(DNA-binding one zinc finger)基因家族是植物特有的一类包含高度保守的C_2-C_2锌指结构域的转录因子,其在植物种子发育与萌发、光、激素以及逆境响应中起到重要调控作用。本研究基于已公布的粗山羊草基因组数据,采用生物信息学方法鉴定了粗山羊草Dof基因,并对其结构、保守结构域、染色体定位、系统进化树和表达谱进行了详细的分析。结果表明:粗山羊草Dof转录因子家族包含10个基因,它们均含有完整的C_2-C_2锌指保守结构域;每个基因含有1~3个内含子,AetDof家族蛋白共包含11个保守的模体;该家族基因除7号染色体外,在其余染色体上均有分布;与拟南芥Dof蛋白构建系统发育树发现,该家族基因可被分为3个亚族;转录组数据分析显示,粗山羊草Dof基因的表达具有组织特异性,其中3个转录因子(AetDof1,AetDof7,AetDof10)在种子中特异性强表达。本研究结果为深入解析粗山羊草Dof基因的功能提供了参考依据。
[Abstract]:The DOF (DNA-binding one zinc finger) gene family is a kind of transcription factor which contains highly conserved C _ 2-C _ 2 zinc finger domain, which plays an important role in the development and germination of plant seeds, light, hormone and stress response. Based on the published genome data of Leymus chinensis, the Dof gene of Leymus chinensis is identified by means of bioinformatics, and its structure, conserved domain, chromosome location, phylogenetic tree and expression profile are analyzed in detail. The results showed that the DOF transcription factor family contained 10 genes, all of which contained a complete C _ 2-C _ 2 zinc finger conserved domain; each gene contained 1 to 3 introns, and the AetDof family protein contained 11 conservative phantom; the family gene, except for chromosome 7, It is found that the gene of the family can be divided into 3 subfamilies and 3 transcription factors (AetDof1, AetDof7, AetDof10) was strongly expressed in the seeds. The results of this study provide a reference for the in-depth analysis of the function of the Dof gene in the Leymus chinensis.
【作者单位】：河南农业大学农学院河南省粮食作物协同创新中心;河南省农业科学院小麦研究所小麦国家工程实验室;
【基金】：国家自然科学基金(31271725) 河南省重大科技专项(161100110500) 河南省科技攻关计划(162102110001) 河南省农业科学院优秀青年科技基金(2016YQ01)共同资助
【分类号】：Q943.2;S512.1

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