PCSK9基因Rs72555377位点多态性和急性冠脉综合的关系

发布时间：2019-04-12 10:23

【摘要】：目的:PCCSK9基因作为第三个高胆固醇血症的遗传学靶点,在脂质代谢中发挥着重要作用,本次研究探讨PCSK9基因Rs72555377位点多态性与急性冠脉综合征(包括ST段抬高性心肌梗塞、非ST段抬高性心肌梗塞、不稳定性心绞痛)的发病关系。方法:收入2014年12月至2016年3月期间在宁波市第一医院住院的急性冠脉综合征病人150例以及期间在宁波市第一医院体检的健康人群150例,经知情同意后收集他们的基本资料、临床生化指标和空腹12小时后的静脉血液3ml。应用PCR技术以及基因测序鉴定PCSK9基因Rs72555377位点上的等位基因。使用SPSS18.0进行统计数据分析,主要包括t检验、方差分析、X2检验等方法。结果:在本次研究中PCSK9基因Rs72555377位点多态性(L9/L9,L9/L10,L10/L10)与急性冠脉综合征的发病没有发现显著相关性(P=0.43)。总体上PCSK9基因Rs72555377位点上L9/L10相比L9/L9它们的HDL-C水平具有显著相关性(P0.01),它们的LDL-C水平没有显著相关性(P=0.257)。结论:在本研究中在PCSK9基因Rs72555377位点共发现L9、L10两种等位基因,其发生频率为0.837和0.163。PCSK9基因Rs72555377位点多态性和急性冠脉综合征的发病没有明显相关;总体上包含L10的具有更高HDL-C水平,所以考虑L10是具有保护性的等位基因。而PCSK9基因Rs72555377位点是否具有更多的等位基因有待进一步大样本的研究。
[Abstract]:Objective: as the third genetic target of hypercholesterolemia, PCCSK9 gene plays an important role in lipid metabolism. This study was to investigate the relationship between Rs72555377 polymorphism of PCSK9 gene and acute coronary syndrome (including ST elevation myocardial infarction). Non-ST segment elevation myocardial infarction, unstable angina pectoris. Methods: 150 patients with acute coronary syndrome admitted to Ningbo first Hospital from December 2014 to March 2016, and 150 healthy people who were examined at Ningbo first Hospital during the period from December 2014 to March 2016, were collected with informed consent. The clinical biochemical indexes and venous blood after 12 hours on an empty stomach were 3ml. PCR technique and gene sequencing were used to identify the alleles at Rs72555377 locus of PCSK9 gene. SPSS18.0 was used for statistical data analysis, including t-test, variance analysis, X2 test and so on. Results: in this study, there was no significant correlation between PCSK9 gene Rs72555377 polymorphism (L9 / L9, L9 / L10, L10 / L10) and the incidence of acute coronary syndrome (P = 0.43). On the whole, L9/L10 at Rs72555377 locus of PCSK9 gene had significant correlation with their HDL-C level compared with L9/L9 (P0.01), but there was no significant correlation between their LDL-C level (P < 0. 257). Conclusion: L9 and L10 alleles were found in the Rs72555377 locus of PCSK9 gene in this study. The frequency of L9 and L10 alleles was 0.837 and the polymorphism of Rs72555377 site of 0.163.PCSK9 gene had no significant correlation with the pathogenesis of acute coronary syndrome (ACS). Generally, L10 had higher HDL-C levels, so L10 was considered to be a protective allele. Whether the Rs72555377 locus of PCSK9 gene has more alleles or not needs to be further studied.
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.4

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