菜豆普通细菌性疫病抗性基因定位

发布时间：2019-04-16 08:39

【摘要】：菜豆普通细菌性疫病是世界上危害普通菜豆生产的最严重的病害之一。龙芸豆5号是我国黑龙江省的主栽品种,对菜豆普通细菌性疫病表现出良好的抗性。为定位来源于龙芸豆5号的抗性基因,本研究构建了包含785个单株的F2分离群体。基于该群体构建了一张包含206个SSR标记,总长度1648.42 c M,标记间平均遗传距离8.00 c M的遗传图谱。图谱包含12个连锁群,各连锁群平均长度137.37 c M,连锁群上标记数量3~35个。结合温室表型鉴定结果,采用QTL Ici Mapping v4.0软件的完备区间作图法进行QTL定位和效应估计。接种14 d后在Pv06染色体上检测到一个抗病QTL。该位点位于标记p6s249与p6s183之间,加性效应值为0.44,说明增效基因来源于龙芸豆5号,LOD值为5.93,表型贡献率为4.61%,该抗病QTL的效应值相对较低,将在培育稳定持久的抗菜豆普通细菌性疫病的品种中发挥作用。最后,对抗性基因紧密连锁的11对SSR引物与菜豆普通细菌性疫病抗性的关联分析表明,SSR标记p6s249与菜豆普通细菌性疫病抗性极显著关联(P0.001),该标记可用于抗病分子育种。
[Abstract]:Common bacterial blight of kidney bean is one of the most serious diseases in the world. Long kidney bean No. 5 is the main cultivar of Heilongjiang Province in China, and it has good resistance to common bacterial blight of kidney bean. In order to locate the resistance gene derived from Longrendou No. 5, a F2 segregation population containing 785 individual plants was constructed in this study. Based on the population, a genetic map consisting of 206 SSR markers with a total length of 1648.42 cm and an average genetic distance of 8.00 cm between markers was constructed. The map contains 12 linkage groups, the average length of each linkage group is 137.37 cm, and the number of markers on linkage group is 3 / 35. Combined with the results of greenhouse phenotypic identification, the complete region mapping method of QTL Ici Mapping v4.0 software was used for QTL localization and effect estimation. A disease-resistant QTL. was detected on the Pv06 chromosome 14 days after inoculation. The locus was located between marker p6s249 and p6s183, and the additive effect value was 0.44, indicating that the synergist gene originated from Longrendou 5, the LOD value was 5.93, the phenotypic contribution rate was 4.61%, and the effective value of the disease resistance QTL was relatively low. It will play a role in the cultivation of stable and lasting varieties resistant to common bacterial blight of kidney bean. Finally, 11 pairs of SSR primers closely linked to the resistance gene to common bacterial blight of kidney bean showed that the SSR marker p6s249 was highly significantly associated with the resistance to common bacterial blight of kidney bean (P0.001). This marker can be used for disease resistance molecular breeding.
【作者单位】：中国农业科学院作物科学研究所;
【基金】：国家现代农业产业技术体系建设专项(CARS-09) 国家科技支撑计划项目(2013BAD01B03-18a) 中国农业科学院科技创新工程项目资助~~
【分类号】：S436.43

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