意大利蜜蜂6日龄幼虫肠道内球囊菌的差异表达基因分析
发布时间：2019-04-16 16:47
【摘要】：为检测蜜蜂球囊菌(Ascosphaera apis)在胁迫意大利蜜蜂(Apis mellifera ligustica,简称意蜂)幼虫后期的基因表达谱,利用RNA-seq技术对纯培养的球囊菌孢子及球囊菌胁迫的意蜂6日龄幼虫肠道进行深度测序。通过比较处理组和对照组得到21 361个差异表达基因(DEGs),包括15 306个上调基因和6 055个下调基因。GO富集分析结果显示,上调基因富集于39个GO条目(term),基因富集数最多的为细胞进程(2 416unigenes)、细胞(2 408 unigenes)和细胞组件(2 408 unigenes);下调基因富集于38个GO term,基因富集数最多的为代谢进程(1 227 unigenes)、细胞进程(1 185 unigenes)和细胞(1 131 unigenes)。KEGG代谢通路富集分析结果显示,上调基因富集在119个通路,其中基因富集数最多的是核糖体(221 unigenes),其次为内质网中蛋白加工(190 unigenes)和内吞作用(177 unigenes);下调基因富集在113个通路上,基因富集数最多的是核糖体(144 unigenes),其次为RNA转运(113 unigenes)和氨基酸生物合成(108 unigenes)。进一步分析发现,富集在MAPK信号通路上的64个基因上调表达,说明该通路在球囊菌胁迫后期被激活。研究结果不仅为揭示球囊菌在胁迫意蜂幼虫后期的病原-宿主互作提供了重要信息,也为阐明球囊菌致病的分子机制奠定了基础。
[Abstract]:In order to detect the gene expression profile of (Ascosphaera apis) in the late larval stage of Italian honeybee (Apis mellifera ligustica, (Apis mellifera), The in-depth sequencing of the 6-day-old larvae of S.aeruginosa under pure culture and 6-day-old stress was carried out by RNA-seq technique. 21,361 differentially expressed genes (DEGs), including 15,306 up-regulated genes and 6,055 down-regulated genes, were obtained from comparison treatment group and control group. Go enrichment analysis showed that the up-regulated genes were enriched in 39 GO entry (term),. The most abundant genes were cell process (2 416unigenes), cell (2 408 unigenes) and cell module (2 408 unigenes);). The down-regulated genes were the most abundant in 38 GO term, genes (1 227 unigenes), cell process (1 185 unigenes) and 1 131 unigenes). The results of KEGG metabolic pathway enrichment analysis showed that the up-regulated genes were enriched in 1 227 unigenes cells and 1 131 unigenes cells, and the up-regulated genes were enriched in 1 227 unigenes cells and 1 131 unigenes cells. The most abundant genes were ribosomes (221 unigenes),) followed by endoplasmic reticulum protein processing (190 unigenes) and endocytosis (177 unigenes);). The down-regulated genes were enriched in 113 pathways, and the most abundant genes were ribosomes (144 unigenes), followed by RNA transport (113 unigenes) and amino acid biosynthesis (108 unigenes). Further analysis showed that 64 genes enriched in the MAPK signaling pathway were up-regulated, indicating that the pathway was activated in the late stage of stress. The results not only provide important information for revealing the pathogen-host interaction in the late stage of stress on Italian bee larvae, but also lay a foundation for elucidating the molecular mechanism of the pathogenesis of B.aeruginosa.
【作者单位】： 福建农林大学蜂学学院;新疆维吾尔自治区蜂业发展中心;
【基金】：现代农业产业技术体系建设专项资金(CARS-45-KXJ7) 福建农林大学科技发展资金(KF2015123) 国家级大学生创新创业项目(201610389016)
【分类号】：S895
[Abstract]:In order to detect the gene expression profile of (Ascosphaera apis) in the late larval stage of Italian honeybee (Apis mellifera ligustica, (Apis mellifera), The in-depth sequencing of the 6-day-old larvae of S.aeruginosa under pure culture and 6-day-old stress was carried out by RNA-seq technique. 21,361 differentially expressed genes (DEGs), including 15,306 up-regulated genes and 6,055 down-regulated genes, were obtained from comparison treatment group and control group. Go enrichment analysis showed that the up-regulated genes were enriched in 39 GO entry (term),. The most abundant genes were cell process (2 416unigenes), cell (2 408 unigenes) and cell module (2 408 unigenes);). The down-regulated genes were the most abundant in 38 GO term, genes (1 227 unigenes), cell process (1 185 unigenes) and 1 131 unigenes). The results of KEGG metabolic pathway enrichment analysis showed that the up-regulated genes were enriched in 1 227 unigenes cells and 1 131 unigenes cells, and the up-regulated genes were enriched in 1 227 unigenes cells and 1 131 unigenes cells. The most abundant genes were ribosomes (221 unigenes),) followed by endoplasmic reticulum protein processing (190 unigenes) and endocytosis (177 unigenes);). The down-regulated genes were enriched in 113 pathways, and the most abundant genes were ribosomes (144 unigenes), followed by RNA transport (113 unigenes) and amino acid biosynthesis (108 unigenes). Further analysis showed that 64 genes enriched in the MAPK signaling pathway were up-regulated, indicating that the pathway was activated in the late stage of stress. The results not only provide important information for revealing the pathogen-host interaction in the late stage of stress on Italian bee larvae, but also lay a foundation for elucidating the molecular mechanism of the pathogenesis of B.aeruginosa.
【作者单位】： 福建农林大学蜂学学院;新疆维吾尔自治区蜂业发展中心;
【基金】：现代农业产业技术体系建设专项资金(CARS-45-KXJ7) 福建农林大学科技发展资金(KF2015123) 国家级大学生创新创业项目(201610389016)
【分类号】：S895
【参考文献】
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