东串猪FUT2基因CDS扩增、序列分析及组织表达谱检测
发布时间：2019-04-22 21:35
【摘要】：猪α-(1,2)岩藻糖转移酶2(FUT2)基因为鞘糖脂生物合成—球系列通路中的重要基因,可能在断奶仔猪抵抗大肠杆菌F18侵染过程中发挥着调控作用。为探究猪FUT2基因的序列结构及其生物学功能,试验采用PCR扩增得到地方猪品种东串猪FUT2基因的CDS全序列,进而预测和分析FUT2基因的蛋白质序列及其功能区域,同时对其在8头35日龄东串断奶仔猪11个组织中的表达水平进行检测与分析。结果显示,FUT2基因的CDS序列全长为1 023bp,共编码340个氨基酸,FUT2蛋白为脂溶性的亲水蛋白,蛋白结构不稳定,该蛋白存在1个跨膜螺旋结构,但不存在信号肽,表明FUT2蛋白为膜蛋白;FUT2蛋白存在2个N-糖基化位点(185和305位氨基酸),无O-糖基化位点,此外该蛋白还存在14个潜在的磷酸化位点,包括6个Ser、2个Thr和6个Tyr,对其功能区域进行分析发现,FUT2蛋白存在1个超级家族保守结构域:FUT1-FUT2-like(58-319位氨基酸);系统进化树结果显示,猪与牛的亲缘关系相对较近,与人、黑猩猩、大鼠和小鼠等亲缘关系相对较远;FUT2基因在东串断奶仔猪11个组织中均有表达,在消化道和免疫组织中表达水平较高。试验结果推测FUT2基因在断奶仔猪抵抗大肠杆菌F18中可能具有一定的作用,且可能是通过合成岩藻糖转移酶间接发挥其抵抗大肠杆菌F18的作用。
[Abstract]:Porcine 伪-(1, 2) fucosyltransferase 2 (FUT2) gene may play a regulatory role in the resistance of weaned piglets to E. coli F18 infection because of the important genes in the glycosyl lipid biosynthesis-globular pathway. In order to investigate the sequence structure and biological function of porcine FUT2 gene, the full CDS sequence of FUT2 gene was amplified by PCR, and the protein sequence and functional region of FUT2 gene were predicted and analyzed. At the same time, its expression level in 11 tissues of 8 35-day-old weanling piglets was detected and analyzed. The results showed that the full-length CDS sequence of FUT2 gene was 1 023 BP, encoding a total of 340 amino acids. The FUT2 protein was a lipid soluble hydrophilic protein, and the structure of the protein was unstable. There was a cross-membrane helix structure in the protein, but no signal peptide. The results showed that FUT2 protein was a membrane protein. There were 2 N-glycosylation sites (185,305 amino acids) and no O-glycosylation sites in FUT2 protein. In addition, there were 14 potential phosphorylation sites in the protein, including 6 Ser,2 Thr and 6 Tyr,. Analysis of its functional domain revealed that there was a superfamily conserved domain of FUT2 protein: FUT1-FUT2-like (amino acid position 58). Phylogenetic tree results showed that the phylogenetic relationship between pigs and cattle was relatively close to that of humans, chimpanzees, rats and mice. FUT2 gene was expressed in 11 tissues of weanling piglets, and the expression level was higher in digestive tract and immune tissues. The results suggest that FUT2 gene may play a certain role in the resistance of weanling piglets to Escherichia coli F18, and may play an indirect role in its resistance to Escherichia coli F18 through the synthesis of fucosyltransferase.
【作者单位】： 扬州大学江苏省动物遗传繁育与分子设计重点实验室;江苏省种猪繁育和健康养殖工程技术研究中心;
【基金】：国家科技支撑计划(2015BAD03B01) 江苏省科技支撑计划(BE2012330) 苏州市科技支撑计划(SNG201410) 太仓市产学研联合创新资金(TC2014CY1)
【分类号】：S828
本文编号：2463214
[Abstract]:Porcine 伪-(1, 2) fucosyltransferase 2 (FUT2) gene may play a regulatory role in the resistance of weaned piglets to E. coli F18 infection because of the important genes in the glycosyl lipid biosynthesis-globular pathway. In order to investigate the sequence structure and biological function of porcine FUT2 gene, the full CDS sequence of FUT2 gene was amplified by PCR, and the protein sequence and functional region of FUT2 gene were predicted and analyzed. At the same time, its expression level in 11 tissues of 8 35-day-old weanling piglets was detected and analyzed. The results showed that the full-length CDS sequence of FUT2 gene was 1 023 BP, encoding a total of 340 amino acids. The FUT2 protein was a lipid soluble hydrophilic protein, and the structure of the protein was unstable. There was a cross-membrane helix structure in the protein, but no signal peptide. The results showed that FUT2 protein was a membrane protein. There were 2 N-glycosylation sites (185,305 amino acids) and no O-glycosylation sites in FUT2 protein. In addition, there were 14 potential phosphorylation sites in the protein, including 6 Ser,2 Thr and 6 Tyr,. Analysis of its functional domain revealed that there was a superfamily conserved domain of FUT2 protein: FUT1-FUT2-like (amino acid position 58). Phylogenetic tree results showed that the phylogenetic relationship between pigs and cattle was relatively close to that of humans, chimpanzees, rats and mice. FUT2 gene was expressed in 11 tissues of weanling piglets, and the expression level was higher in digestive tract and immune tissues. The results suggest that FUT2 gene may play a certain role in the resistance of weanling piglets to Escherichia coli F18, and may play an indirect role in its resistance to Escherichia coli F18 through the synthesis of fucosyltransferase.
【作者单位】： 扬州大学江苏省动物遗传繁育与分子设计重点实验室;江苏省种猪繁育和健康养殖工程技术研究中心;
【基金】：国家科技支撑计划(2015BAD03B01) 江苏省科技支撑计划(BE2012330) 苏州市科技支撑计划(SNG201410) 太仓市产学研联合创新资金(TC2014CY1)
【分类号】：S828
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，本文编号：2463214
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