K-ras基因状态及ras蛋白表达在胃印戒细胞癌免疫表型分类中的意义

发布时间：2019-04-25 11:18

【摘要】：目的探讨K-ras基因状态及ras蛋白表达在胃印戒细胞癌免疫表型分类中的意义。方法应用组织芯片免疫组织化学技术检测180例胃印戒细胞癌组织中ras蛋白的表达;PCR扩增和DNA直接测序法检测癌组织中K-ras基因12、13密码子的突变情况。结果 ras蛋白阳性50例(27.8%),ras蛋白阳性表达率在肠型印戒细胞癌中显著高于胃型和混合型印戒细胞癌(P0.05)。在淋巴结转移阳性组织中,ras蛋白阳性表达率明显高于无淋巴结转移组织(P0.05);随着肿瘤侵袭、演进及临床分期的升高,ras蛋白阳性表达率明显增高(P0.05)。K-ras基因突变型22例(12.2%)。所有突变位点均位于12密码子,突变类型包括:GGT→AGT 1例,GGT→TGT 1例,GGT→GCT 2例,GGT→GTT 8例,GGT→GAT 10例。13密码子未发现突变。肠型印戒细胞癌K-ras基因突变率显著高于胃型和混合型印戒细胞癌(P0.05)。K-ras基因突变型病例ras蛋白阳性表达率明显高于K-ras基因野生型病例(P0.05)。Spearman等级相关分析显示,ras蛋白表达与K-ras基因突变呈正相关(r=0.61,P0.05)。结论 ras蛋白的过表达可能与胃印戒细胞癌侵袭潜能、淋巴结转移相关,ras蛋白表达和K-ras基因突变与肿瘤的免疫表型变化存在相关性,它们在癌细胞表型转化过程中可能起到一定作用。
[Abstract]:Objective to investigate the significance of K-ras gene status and ras protein expression in the classification of immunophenotype in signet ring cell carcinoma of stomach. Methods the expression of ras protein was detected by tissue chip immunohistochemistry in 180 cases of signet ring cell carcinoma of the stomach, and the mutations of codon 12 and 13 of K-ras gene were detected by PCR amplification and DNA direct sequencing. Results the positive rate of ras protein in intestinal signet ring cell carcinoma was significantly higher than that in gastric signet ring cell carcinoma and mixed signet ring cell carcinoma (P 0.05). The positive rate of), ras protein was 27.8% in intestinal signet ring cell carcinoma. The positive expression rate of ras protein in lymph node metastasis positive tissues was significantly higher than that in non lymph node metastasis tissues (P0.05). With the increase of tumor invasion, progression and clinical stage, the positive expression rate of ras protein was significantly increased (P0.05), and 22 cases (12.2%) of K-ras gene mutation were found. All the mutation sites were located at codon 12, including 1 case of GGT / AGT, 1 case of GGT / TGT, 2 cases of GGT / GCT, 8 cases of GGT / GTT and 10 cases of GGT / GAT. No mutation was found in codon 13. The mutation rate of K-ras gene in intestinal signet ring cell carcinoma was significantly higher than that in gastric signet ring cell carcinoma and mixed signet ring cell carcinoma (P0.05). The positive expression rate of ras protein in K-ras mutant was significantly higher than that in K-ras wild type (P0). .05). Spearman rank correlation analysis, The expression of ras protein was positively correlated with the mutation of K-ras gene (r = 0.61, P0.05). Conclusion the overexpression of ras protein may be associated with invasion potential and lymph node metastasis of signet ring cell carcinoma. The expression of ras protein and mutation of K-ras gene may be related to the changes of immunophenotype of gastric signet ring cell carcinoma. They may play a role in the process of phenotypic transformation of cancer cells.
【作者单位】：南昌大学第一附属医院病理科;江西省南昌市洪都中医院急诊科;
【基金】：江西省卫生计生委科技计划项目(20155179)
【分类号】：R735.2

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